Hajime Wada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
2 protocols

Shin-ya Miyagishima Department of Cell Genetics, National Institute of Genetics, Japan
2 protocols
Agnieszka Zienkiewicz Nicolaus Copernicus University
23 protocols

Ali Kermani University of Michigan, Ann Arbor
8 protocols

Arsalan Daudi University of California
157 protocols

Baohua Li University of California, Davis
4 protocols

Reviewer
Kumiko Okazaki
  • Assistant professor, Hiroshima University
Research focus
  • Plant science
  • 2 Author merit

Education

Ph.D. (Science), The University of Tokyo

2 Protocols published
Preparation of Chloroplast Lipid Membrane and Lipid-protein Interaction Assay
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 7641, Q&A: 0
Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), ...
Measurement of PI4P Levels in Intact Chloroplasts Isolated from Arabidopsis thaliana
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 7663, Q&A: 1
Phosphatidylinositol 4-phosphate (PI4P), a major species of phosphoinositides, modulates many fundamental cellular processes. We have recently revealed that PI4P plays an important role in chloroplast division as a negative regulator. Despite its ...
5 Protocols reviewed
A Detailed Protocol for Preparing Millimeter-sized Supergiant Liposomes that Permit Efficient Eukaryotic Cell-free Translation in the Interior
Authors:  Hajime Takahashi and Atsushi Ogawa, date: 06/20/2021, view: 728, Q&A: 0

Liposomes have been used as a pseudo cell membrane for encapsulating biomolecules and creating an artificial cell in the interior where biochemical reactions can occur. Among the several methods used to prepare biomolecule-encapsulating liposomes,

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Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves
Authors:  Hari Kiran Kotapati and Philip D. Bates, date: 12/20/2020, view: 910, Q&A: 0

Plant lipid metabolism is a dynamic network where synthesis of essential membrane lipids overlaps with synthesis of valuable storage lipids (e.g., vegetable oils). Monogalactosyldiacylglycerol (MGDG) is a key component of the chloroplast membrane

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