Hajime Wada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
2 protocols

Shin-ya Miyagishima Department of Cell Genetics, National Institute of Genetics, Japan
2 protocols
Arsalan Daudi University of California
157 protocols

Baohua Li University of California, Davis
4 protocols

Dr. Ali Anwar Seoul National University
1 protocol

Jose Reyes-Darias Vircell S.L.
9 protocols

Reviewer
Kumiko Okazaki
  • Assistant professor, Hiroshima University
Research focus
  • Plant science
  • 2 Author merit

Education

Ph.D. (Science), The University of Tokyo

2 Protocols published
Preparation of Chloroplast Lipid Membrane and Lipid-protein Interaction Assay
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 6727, Q&A: 0
Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), ...
Measurement of PI4P Levels in Intact Chloroplasts Isolated from Arabidopsis thaliana
Authors:  Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada, date: 02/05/2016, view: 6768, Q&A: 1
Phosphatidylinositol 4-phosphate (PI4P), a major species of phosphoinositides, modulates many fundamental cellular processes. We have recently revealed that PI4P plays an important role in chloroplast division as a negative regulator. Despite its ...
3 Protocols reviewed
Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
Authors:  Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 652, Q&A: 0
Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered ...
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A Sensitive Coupled Enzyme Assay for Measuring Kinase and ATPase Kinetics Using ADP-Specific Hexokinase
Authors:  Ciaran R. McFarlane and James W. Murray, date: 05/05/2020, view: 1169, Q&A: 0
Kinases and ATPases perform essential biological functions in metabolism and regulation. Activity of these enzymes is commonly measured by coupling ATP consumption to the synthesis of a detectable product. For most assay systems the ATP ...
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