Rachael Giersch Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
1 protocol

Jeremy Thorner University of California, Berkeley
1 protocol
Joëlle Schläpfer University of Zurich
13 protocols

Melike Çağlayan University of Florida
4 protocols

Ralph Boettcher Max Planck Institute for Biochemistry
50 protocols

Reviewer
Gregory C. Finnigan
  • Faculty, Kansas State University
Research focus
  • Molecular biology
  • 2 Author merit

Education

University of Oregon

Lab information

https://www.k-state.edu/bmb/department/directory/finnigan/lab/

2 Protocols published
酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法
Authors:  Rachael M. Giersch and Gregory C. Finnigan, date: 09/20/2017, view: 9236, Q&A: 0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
通过同源重组进行复合体的体内连接及在酿酒酵母中进行高效质粒拯救
Authors:  Gregory C. Finnigan and Jeremy Thorner, date: 07/05/2015, view: 10375, Q&A: 0
The protocols presented here allow for the facile generation of a wide variety of complex multipart DNA constructs (tagged gene products, gene fusions, chimeric proteins, and other variants) using homologous recombination and in vivo ...
2 Protocols reviewed
Trypsin Sensitivity Assay to Study the Folding Status of Proteins
Authors:  Satoshi Ninagawa and Kazutoshi Mori, date: 10/05/2016, view: 8525, Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing trypsin sensitivity. Unfolded/misfolded proteins are more susceptible to trypsin than folded proteins, because trypsin easily accesses and cleaves loosely folded parts of proteins. ...
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PNGase Sensitivity Assay to Study the Folding Status of Proteins
Authors:  Satoshi Ninagawa and Kazutoshi Mori, date: 10/05/2016, view: 6252, Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme. N-glycans on unfolded/misfolded proteins are more susceptible to PNGase than ...
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