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Coauthors
Rachael Giersch Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
1 protocol

Jeremy Thorner Division of Biochemistry, Biophysics and Structural Biology, Department of Molecular and Cell Biology, University of California, USA
1 protocol
Coreviewers
Joëlle Schlapfer Lawrence Berkeley National Laboratory
8 protocols

Ralph Boettcher Max Planck Institute for Biochemistry
33 protocols

Reviewer
Gregory C. Finnigan
  • Faculty, Kansas State University
Research focus
  • Molecular biology
  • 2 Author merit
2 Protocols published
Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
Authors:  Rachael M. Giersch and Gregory C. Finnigan, date: 09/20/2017, view: 3749, Q&A: 0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
Complex in vivo Ligation Using Homologous Recombination and High-efficiency Plasmid Rescue from Saccharomyces cerevisiae
Authors:  Gregory C. Finnigan and Jeremy Thorner, date: 07/05/2015, view: 5040, Q&A: 0
The protocols presented here allow for the facile generation of a wide variety of complex multipart DNA constructs (tagged gene products, gene fusions, chimeric proteins, and other variants) using homologous recombination and in vivo ...
2 Protocols reviewed
Trypsin Sensitivity Assay to Study the Folding Status of Proteins
Authors:  Satoshi Ninagawa and Kazutoshi Mori, date: 10/05/2016, view: 3585, Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing trypsin sensitivity. Unfolded/misfolded proteins are more susceptible to trypsin than folded proteins, because trypsin easily accesses and cleaves loosely folded parts of proteins. ...
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PNGase Sensitivity Assay to Study the Folding Status of Proteins
Authors:  Satoshi Ninagawa and Kazutoshi Mori, date: 10/05/2016, view: 2961, Q&A: 0
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme. N-glycans on unfolded/misfolded proteins are more susceptible to PNGase than ...
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