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Rachael Giersch
Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
1 protocol
Jeremy Thorner
University of California, Berkeley
1 protocol
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Joëlle Schläpfer
University of Zurich
13 protocols
Melike Çağlayan
University of Florida
4 protocols
Ralph Boettcher
Max Planck Institute for Biochemistry
47 protocols
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Reviewer
Gregory C. Finnigan
Faculty, Kansas State University
Research focus
Molecular biology
Contributions
2
Author merit
Reviewer merit
Editor merit
Lab information
https://www.k-state.edu/bmb/department/directory/finnigan/lab/
2 Protocols published
Method for Multiplexing CRISPR/Cas9 in
Saccharomyces cerevisiae
Using Artificial Target DNA Sequences
Authors:
Rachael M. Giersch
and
Gregory C. Finnigan
,
date:
09/20/2017,
view:
8174,
Q&A:
0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
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Complex
in vivo
Ligation Using Homologous Recombination and High-efficiency Plasmid Rescue from
Saccharomyces cerevisiae
Authors:
Gregory C. Finnigan
and
Jeremy Thorner
,
date:
07/05/2015,
view:
9274,
Q&A:
0
The protocols presented here allow for the facile generation of a wide variety of complex multipart DNA constructs (tagged gene products, gene fusions, chimeric proteins, and other variants) using homologous recombination and
in vivo
...
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2 Protocols reviewed
Trypsin Sensitivity Assay to Study the Folding Status of Proteins
Authors:
Satoshi Ninagawa
and
Kazutoshi Mori
,
date:
10/05/2016,
view:
7524,
Q&A:
0
This protocol aims to evaluate folding status of proteins, utilizing trypsin sensitivity. Unfolded/misfolded proteins are more susceptible to trypsin than folded proteins, because trypsin easily accesses and cleaves loosely folded parts of proteins. ...
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PNGase Sensitivity Assay to Study the Folding Status of Proteins
Authors:
Satoshi Ninagawa
and
Kazutoshi Mori
,
date:
10/05/2016,
view:
5586,
Q&A:
0
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme. N-glycans on unfolded/misfolded proteins are more susceptible to PNGase than ...
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