Aksiniya Asenova University of California, Davis
12 protocols

Antoine de Morree Stanford university
33 protocols

Emilia Krypotou Yale University
9 protocols

Emilie Battivelli Buck institute
5 protocols

Editor
Emily K Cope
  • Northern Arizona University
Research focus
  • Microbiology
6 Protocols reviewed
MNase Digestion for Nucleosome Mapping in Neurospora
Authors:  Cigdem Sancar, Gencer Sancar and Michael Brunner, date: 06/05/2016, view: 4647, Q&A: 0
Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase ...
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Whole Genome Bisulfite Sequencing and DNA Methylation Analysis from Plant Tissue
Authors:  Daniela Pignatta, George W. Bell and Mary Gehring, date: 02/20/2015, view: 11855, Q&A: 0
This protocol describes whole genome bisulfite-sequencing library preparation from plant tissue and subsequent data analysis. Allele-specific methylation analysis and genome-wide identification of differentially methylated regions are additional ...
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5 Protocols edited
Assessing Yeast Cell Survival Following Hydrogen Peroxide Exposure
Authors:  Khoa Tran and Erin M. Green, date: 01/20/2019, view: 834, Q&A: 0
In the presence of oxidative stress, cellular defense systems that can detoxify reactive oxygen species are activated through multiple signaling cascades and transcriptional reprogramming. The budding yeast Saccharomyces cerevisiae has ...
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Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages
Authors:  Victor Jimenez Jr and Fernando P Monroy, date: 01/20/2019, view: 584, Q&A: 0
Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. While pulmonary related infections can occur in healthy humans, binge alcohol use is recognized as a major ...
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