Ryan Cupo
1 protocol

Lin Guo Biochemistry and Molecular Biology, Thomas Jefferson University, 2019-
1 protocol

Emma Robinson Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 2019-2020
1 protocol

James Shorter
  • Professor, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, USA
Research focus
  • Biochemistry
  • 2 Author merit

Education

Ph.D., University of London, U.K.

Publications

2 Protocols published
重组Skd3(人ClpB)蛋白和烟草蚀刻病毒(TEV)蛋白酶在大肠杆菌中的表达与纯化
Authors:  Ryan R. Cupo and James Shorter, date: 12/05/2020, view: 1846, Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

...
核转运蛋白-β2抑制和ALS / FTD相关蛋白FUS的逆转聚集和液-液相分离
Authors:  Emma Robinson, James Shorter and Lin Guo, date: 08/20/2020, view: 2216, Q&A: 0
The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.