Olga Petrova Biological Sciences Department, Binghamton University, State University of New York at Binghamton, USA
1 protocol

Ankita Roy Biological Sciences Department, Binghamton University, State University of New York at Binghamton, USA
1 protocol

Karin Sauer
  • Biological Sciences Department, Binghamton University, State University of New York at Binghamton, USA
Research focus
  • Microbiology
  • 1 Author merit


Ph.D. in Microbiology & Biochemistry, Max-Planck-Institute for Terrestrial Microbiology, Marburg, Germany, 1999

Current position

Professor, Department of Biological Sciences, Binghamton University, Binghamton, NY


  1. Chambers, J. R. and Sauer, K. (2013). Small RNAs and their role in biofilm formation. Trends Microbiol 21(1): 39-49.
  2. Liao, J., Schurr, M. J. and Sauer, K. (2013). The MerR-Like Regulator BrlR Confers Biofilm Tolerance by Activating Multidrug Efflux Pumps in Pseudomonas aeruginosa Biofilms. J Bacteriol 195(15): 3352-3363.
  3. Li, Y., Heine, S., Entian, M., Sauer, K. and Frankenberg-Dinkel, N. (2013). NO-induced biofilm dispersion in Pseudomonas aeruginosa is mediated by a MHYT-domain coupled phosphodiesterase. J Bacteriol.
  4. Adriana Compagnoni, A., M. Dezani-Ciancaglini, P. Giannini, K. Sauer, V. Sharma, and A. Troina. (2012). Parallel BioScape: A Stochastic and Parallel Language for Mobile and Spatial Interactions. In B. Aman and G. Ciobanu, editors, Proceedings of The 6th Workshop on Membrane Computing and Biologically Inspired Process Calculi. Electronic Proceedings in Theoretical Computer Science. (EPTCS) 100: 101-106.
  5. Petrova, O. E. and Sauer, K. (2012). Dispersion by Pseudomonas aeruginosa requires an unusual posttranslational modification of BdlA. Proc Natl Acad Sci U S A 109(41): 16690-16695.
  6. Petrova, O. E. and Sauer, K. (2012). PAS domain residues and prosthetic group involved in BdlA-dependent dispersion response by Pseudomonas aeruginosa biofilms. J Bacteriol 194(21): 5817-5828.
  7. Petrova, O. E., Schurr, J. R., Schurr, M. J. and Sauer, K. (2012). Microcolony formation by the opportunistic pathogen Pseudomonas aeruginosa requires pyruvate and pyruvate fermentation. Mol Microbiol 86(4): 819-835.
  8. Liao, J. and Sauer, K. (2012). The MerR-like transcriptional regulator BrlR contributes to Pseudomonas aeruginosa biofilm tolerance. J Bacteriol 194(18): 4823-4836.
  9. Roy, A. B., Petrova, O. E. and Sauer, K. (2012). The phosphodiesterase DipA (PA5017) is essential for Pseudomonas aeruginosa biofilm dispersion. J Bacteriol 194(11): 2904-2915.
  10. Petrova, O. E. and Sauer, K. (2012). Sticky situations: key components that control bacterial surface attachment. J Bacteriol 194(10): 2413-2425.
  11. Petrova, O. E. and Sauer, K. (2011). SagS contributes to the motile-sessile switch and acts in concert with BfiSR to enable Pseudomonas aeruginosa biofilm formation. J Bacteriol 193(23): 6614-6628.
  12. Petrova, O. E., Schurr, J. R., Schurr, M. J. and Sauer, K. (2011). The novel Pseudomonas aeruginosa two-component regulator BfmR controls bacteriophage-mediated lysis and DNA release during biofilm development through PhdA. Mol Microbiol 81(3): 767-783.
  13. Petrova, O. E. and Sauer, K. (2010). The novel two-component regulatory system BfiSR regulates biofilm development by controlling the small RNA rsmZ through CafA. J Bacteriol 192(20): 5275-5288.
  14. Sanchez, C. J., Shivshankar, P., Stol, K., Trakhtenbroit, S., Sullam, P. M., Sauer, K., Hermans, P. W. and Orihuela, C. J. (2010). The pneumococcal serine-rich repeat protein is an intra-species bacterial adhesin that promotes bacterial aggregation in vivo and in biofilms. PLoS Pathog 6(8): e1001044.
  15. Lizcano, A., Chin, T., Sauer, K., Tuomanen, E. I. and Orihuela, C. J. (2010). Early biofilm formation on microtiter plates is not correlated with the invasive disease potential of Streptococcus pneumoniae. Microb Pathog 48(3-4): 124-130.
  16. Petrova, O. E. and Sauer, K. (2009). A novel signaling network essential for regulating Pseudomonas aeruginosa biofilm development. PLoS Pathog 5(11): e1000668.
  17. Hassett, D. J., Korfhagen, T. R., Irvin, R. T., Schurr, M. J., Sauer, K., Lau, G. W., Sutton, M. D., Yu, H. and Hoiby, N. (2010). Pseudomonas aeruginosa biofilm infections in cystic fibrosis: insights into pathogenic processes and treatment strategies. Expert Opin Ther Targets 14(2): 117-130.
  18. Sauer, K., Steczko, J. and Ash, S. R. (2009). Effect of a solution containing citrate/Methylene Blue/parabens on Staphylococcus aureus bacteria and biofilm, and comparison with various heparin solutions. J Antimicrob Chemother 63(5): 937-945.
  19. Sauer, K., Thatcher, E., Northey, R. and Gutierrez, A. A. (2009). Neutral super-oxidised solutions are effective in killing P. aeruginosa biofilms. Biofouling 25(1): 45-54.
  20. Ash. S.R., J. Steczko, K. Sauer, D.E. Nivens, and L. Brewer. 2008. Bacterial eradication in existing microbial biofilms by zuragen (Methylene blue, citrate, antiseptic solution). International Journal of artificial organs 31: 597-598.
  21. Allegrucci, M. and Sauer, K. (2008). Formation of Streptococcus pneumoniae non-phase-variable colony variants is due to increased mutation frequency present under biofilm growth conditions. J Bacteriol 190(19): 6330-6339.
  22. Platt, M. D., Schurr, M. J., Sauer, K., Vazquez, G., Kukavica-Ibrulj, I., Potvin, E., Levesque, R. C., Fedynak, A., Brinkman, F. S., Schurr, J., Hwang, S. H., Lau, G. W., Limbach, P. A., Rowe, J. J., Lieberman, M. A., Barraud, N., Webb, J., Kjelleberg, S., Hunt, D. F. and Hassett, D. J. (2008). Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions. J Bacteriol 190(8): 2739-2758.
  23. Morici, L. A., Carterson, A. J., Wagner, V. E., Frisk, A., Schurr, J. R., Honer zu Bentrup, K., Hassett, D. J., Iglewski, B. H., Sauer, K. and Schurr, M. J. (2007). Pseudomonas aeruginosa AlgR represses the Rhl quorum-sensing system in a biofilm-specific manner. J Bacteriol 189(21): 7752-7764.
  24. Allegrucci, M. and Sauer, K. (2007). Characterization of colony morphology variants isolated from Streptococcus pneumoniae biofilms. J Bacteriol 189(5): 2030-2038.
  25. Sauer, K., A.H. Rickard., and D.G. Davies. (2007). Biofilms and biocomplexity. ASM Microbe 2 (7): 347-353.
  26. Morgan, R., Kohn, S., Hwang, S. H., Hassett, D. J. and Sauer, K. (2006). BdlA, a chemotaxis regulator essential for biofilm dispersion in Pseudomonas aeruginosa. J Bacteriol 188(21): 7335-7343.
  27. Allegrucci, M., Hu, F. Z., Shen, K., Hayes, J., Ehrlich, G. D., Post, J. C. and Sauer, K. (2006). Phenotypic characterization of Streptococcus pneumoniae biofilm development. J Bacteriol 188(7): 2325-2335.
  28. Southey-Pillig, C. J., Davies, D. G. and Sauer, K. (2005). Characterization of temporal protein production in Pseudomonas aeruginosa biofilms. J Bacteriol 187(23): 8114-8126.
  29. Patrauchan, M. A., Sarkisova, S., Sauer, K. and Franklin, M. J. (2005). Calcium influences cellular and extracellular product formation during biofilm-associated growth of a marine Pseudoalteromonas sp. Microbiology 151(Pt 9): 2885-2897.
  30. Sauer, K., Cullen, M. C., Rickard, A. H., Zeef, L. A., Davies, D. G. and Gilbert, P. (2004). Characterization of nutrient-induced dispersion in Pseudomonas aeruginosa PAO1 biofilm. J Bacteriol 186(21): 7312-7326.
  31. Sauer, K. (2003). The genomics and proteomics of biofilm formation. Genome Biol 4(6): 219.
  32. Sauer, K., Camper, A. K., Ehrlich, G. D., Costerton, J. W. and Davies, D. G. (2002). Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol 184(4): 1140-1154.
  33. Stoodley, P., Sauer, K., Davies, D. G. and Costerton, J. W. (2002). Biofilms as complex differentiated communities. Annu Rev Microbiol 56: 187-209.
  34. Sauer, K. and Camper, A. K. (2001). Characterization of phenotypic changes in Pseudomonas putida in response to surface-associated growth. J Bacteriol 183(22): 6579-6589.
  35. Sauer, K. and Thauer, R. K. (2000). Methyl-coenzyme M formation in methanogenic archaea. Involvement of zinc in coenzyme M activation. Eur J Biochem 267(9): 2498-2504.
  36. Sauer, K. and Thauer, R. K. (1999). Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin. Eur J Biochem 261(3): 674-681.
  37. Sauer, K., and R.K. Thauer. 1999. The role of corrinoids in methanogenesis. In: Chemistry and Biochemistry of B12 (Banerjee, R., Ed.), pp. 655-679, John Wiley & Sons, Inc., New York.
  38. Sauer, K. and Thauer, R. K. (1998). Methanol:coenzyme M methyltransferase from Methanosarcina barkeri--identification of the active-site histidine in the corrinoid-harboring subunit MtaC by site-directed mutagenesis. Eur J Biochem 253(3): 698-705.
  39. Sauer, K. and Thauer, R. K. (1998). His84 rather than His35 is the active site histidine in the corrinoid protein MrtA of the energy conserving methyltransferase complex from Methanobacterium thermoautotrophicum. FEBS Lett 436(3): 401-402.
  40. Asakawa, S., Sauer, K., Liesack, W. and Thauer, R. K. (1998). Tetramethylammonium:coenzyme M methyltransferase system from methanococcoides sp. Arch Microbiol 170(4): 220-226.
  41. Sauer, K. and Thauer, R. K. (1997). Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction. Eur J Biochem 249(1): 280-285.
  42. Sauer, K., Harms, U. and Thauer, R. K. (1997). Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1. Eur J Biochem 243(3): 670-677.
1 Protocol published
Extraction and Quantification of Cyclic Di-GMP from Pseudomonas aeruginosa
Authors:  Ankita Basu Roy, Olga E. Petrova and Karin Sauer, date: 07/20/2013, view: 11121, Q&A: 1
Cyclic di-GMP (c-di-GMP) has emerged as an important intracellular signaling molecule, controlling the transitions between planktonic (free-living) and sessile lifestyles, biofilm formation, and virulence in a wide variety of microorganisms. The ...
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