Nigel Temperton Vial Pseudotype Unit, Medway School of Pharmacy, University of Kent, United Kingdom, United Kingdom,
2 protocols

Fabrizio Biuso 1 protocol

Eleonora Molesti 1 protocol
Jeremy Welsch University of Michigan, Medical School
3 protocols

Longping Tse University of North Carolina at Chapel Hill
27 protocols

Masfique Mehedi University of North Dakota
3 protocols

Ran Chen Zhejiang JFK Biological Technology Co. Ltd.
5 protocols

Reviewer
George William Carnell
  • Post-Doc, University of Cambridge
Research focus
  • Immunology
  • 2 Author merit

Education

PhD, University of Kent, 2018

2 Protocols published
A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
Authors:  Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 4662, Q&A: 0
This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
Authors:  George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 11582, Q&A: 1
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...
4 Protocols reviewed
Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike

The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

...
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Production of Quantum Dots-containing Influenza Virus Particles for Studying Viral Uncoating Processes
Authors:  Chong Qin, Xiaowei Zhang and Zongqiang Cui, date: 09/20/2019, view: 3172, Q&A: 0
The genome of influenza A virus (IAV) comprises eight pinlike genomic segments called vRNPs enclosed in viral capsid. During infection, uncoating is the key step for viral replication and represents an antiviral therapeutic target, but it is ...
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