Anne Ephrussi Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), Germany
2 protocols

Frank Wippich Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Germany, 69117 Germany,
1 protocol
Abhijit Kale Buck Institute for Research on Aging
8 protocols

Alessandro Didonna University of California, San Francisco
27 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
76 protocols

Jihyun Kim Finless Foods
19 protocols

Editor
Imre Gáspár
  • Faculty, INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna
Research focus
  • Molecular biology
  • RNA localization, mRNP assembly, cytoskeletal transport,
    meiosis, chromatin remodelling during development
  • 2 Author merit

Education

PhD, University of Szeged, 2009

Lab information

Kikue Tachibana Lab
https://www.imba.oeaw.ac.at/research/kikue-tachibana/

Publications

https://scholar.google.de/citations?user=I2gbMMwAAAAJ&hl=en
https://scholar.google.de/citations?user=I2gbMMwAAAAJ&hl=en
2 Protocols published
Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
Authors:  Imre Gaspar, Frank Wippich and Anne Ephrussi, date: 03/05/2018, view: 4784, Q&A: 0
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using ...
Ex vivo Ooplasmic Extract from Developing Drosophila Oocytes for Quantitative TIRF Microscopy Analysis
Authors:  Imre Gáspár and Anne Ephrussi, date: 07/05/2017, view: 4394, Q&A: 0
Understanding the dynamic behavior and the continuously changing composition of macromolecular complexes, subcellular structures and organelles is one of areas of active research in both cell and developmental biology, as these changes directly ...
3 Protocols reviewed
A Novel Hygrotaxis Assay for Assessing Thirst Perception and Water Sensation in Drosophila
Authors:  Yuanjie Sun, Shan Gao, Feiteng Ji and Yan Zhu, date: 02/05/2019, view: 1178, Q&A: 0
Water is essential for the survival of terrestrial animals. Animals obtain water from their food, from metabolism and, more importantly, by drinking. It is therefore vital for dehydrated animals to efficiently locate water sources in the wild. ...
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Dual-probe RNA FRET-FISH in Yeast
Authors:  Gable M. Wadsworth, Rasesh Y. Parikh and Harold D. Kim, date: 06/05/2018, view: 3192, Q&A: 0
mRNA Fluorescence In Situ Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can ...
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4 Protocols edited
Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation
The purification of nucleic acids is one of the most common procedures employed in modern molecular biology laboratories. Typically, commercial column-based protocols are utilized to isolate DNA or RNA from various sources. However, these methods ...
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Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
Authors:  I-Ting Teng, Xiangning Bu and Inhee Chung, date: 09/20/2019, view: 483, Q&A: 0
Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to ...
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