Alba Blesa Universidad Francisco de Vitoria
15 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
13 protocols

Dennis Nürnberg Imperial College London
47 protocols

Fanglian He Bio-protocol
205 protocols

Reviewer
Seda Ekici
  • University of Washington
Research focus
  • Microbiology
8 Protocols reviewed
Measurement of ATPase Activity of Valosin-containing Protein/p97
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was ...
More >
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:  Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez, date: 01/05/2018, view: 5497, Q&A: 0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
More >
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.