Manuel D Gahete —BIO-PROTOCOL

Home About Us For Authors Submit Protocol Archive

Justo Castaño Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols

Raúl Luque Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols

Daniel Kraus Würzburg University Hospital
6 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
56 protocols

Imre Gáspár INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna
3 protocols

Kae-Jiun Chang University of California, San Francisco
11 protocols

Reviewer

Manuel D Gahete

Post-Doc, IMIBIC - Maimonides Institute for Biomedical Research of Córdoba

Research focus

Cancer biology

2 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Cordoba, 2010

2 Protocols published

Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells

Authors: Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 8592, Q&A: 0

This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino ...

More >

GPCRs Interaction Measurement by Fluorescence Resonance Energy Transfer (FRET)

Authors: Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 6458, Q&A: 0

This is a protocol to determine the physical interaction of a G-protein coupled receptor (GPCR) with itself (homodimerization) or with other GPCR (heterodimerazation) using fluorescence resonance energy transfer (FRET). FRET is a distance-dependent ...

More >

6 Protocols reviewed

Dual-probe RNA FRET-FISH in Yeast

Authors: Gable M. Wadsworth, Rasesh Y. Parikh and Harold D. Kim, date: 06/05/2018, view: 2163, Q&A: 0

mRNA Fluorescence In Situ Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can ...

More >

FRET-based Stoichiometry Measurements of Protein Complexes in vitro

Authors: Francesca Mattiroli, Yajie Gu and Karolin Luger, date: 02/05/2018, view: 2593, Q&A: 0

For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry ...

More >

More protocols >