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A peer-reviewed protocol journal. No publication fee; no access fee.
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Justo Castaño
Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols
Raúl Luque
Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols
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Daniel Kraus
Würzburg University Hospital
6 protocols
Gal Haimovich
Molecular Genetics, Weizmann Institute of Science, Israel
48 protocols
Imre Gáspár
INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna
3 protocols
Kae-Jiun Chang
University of California, San Francisco
11 protocols
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Reviewer
Manuel D Gahete
Post-Doc, IMIBIC - Maimonides Institute for Biomedical Research of Córdoba
Research focus
Cancer biology
Contributions
2
Author merit
Reviewer merit
Editor merit
Education
PhD, University of Cordoba, 2010
2 Protocols published
Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells
Authors:
Manuel D. Gahete
,
Raúl M. Luque
and
Justo P. Castaño
,
date:
11/20/2012,
view:
8256,
Q&A:
0
This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino ...
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GPCRs Interaction Measurement by Fluorescence Resonance Energy Transfer (FRET)
Authors:
Manuel D. Gahete
,
Raúl M. Luque
and
Justo P. Castaño
,
date:
11/20/2012,
view:
6177,
Q&A:
0
This is a protocol to determine the physical interaction of a G-protein coupled receptor (GPCR) with itself (homodimerization) or with other GPCR (heterodimerazation) using fluorescence resonance energy transfer (FRET). FRET is a distance-dependent ...
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6 Protocols reviewed
Dual-probe RNA FRET-FISH in Yeast
Authors:
Gable M. Wadsworth
,
Rasesh Y. Parikh
and
Harold D. Kim
,
date:
06/05/2018,
view:
1785,
Q&A:
0
mRNA Fluorescence
In Situ
Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can ...
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FRET-based Stoichiometry Measurements of Protein Complexes
in vitro
Authors:
Francesca Mattiroli
,
Yajie Gu
and
Karolin Luger
,
date:
02/05/2018,
view:
2136,
Q&A:
0
For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry
...
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