Agnès Groisillier Sorbonne Universités, University Pierre and Marie Curie
3 protocols

Alessandro Didonna University of California, San Francisco
19 protocols

André Grassmann University of Connecticut Health Center
1 protocol

Angela Corona University of Cagliari
3 protocols

Editor
Yanjie Li
  • Yale University
Research focus
  • Biochemistry
  • 0 Author merit

Education

Ph.D., Department of Chemistry, University of Toronto, Canada, 2010

Current position

Postdoc Associate, Department of Molecular Biophysics and Biochemistry, Yale University, USA

Publications (selected)

  1. Li, X.*, Li, Y.*, Arendt, C. S. and Hochstrasser, M. (2016). Distinct elements in the proteasomal β5 subunit propeptide required for autocatalytic processing and poteasome assembly. J Biol Chem 291(4): 1991-2003. (*Equal contribution)
  2. Li, Y., Tomko, R. J. Jr. and Hochstrasser, M. (2015). Proteasomes: Isolation and activity assays. Curr Protoc Cell Biol 67: 3 43 41-20.
  3. Wang, S. C., Li, Y., Ho, M., Bernal, M. E., Sydor, A. M., Kagzi, W. R. and Zamble, D. B. (2010). The response of Escherichia coli NikR to nickel: a second nickel-binding site. Biochemistry 49(31): 6635-6645.
  4. Wang, S. C., Li, Y., Robinson, C. V. and Zamble, D. B. (2010). Potassium is critical for the Ni(II)-responsive DNA-binding activity of Escherichia coli NikR. J Am Chem Soc 132(5): 1506-1507.
  5. Li, Y. and Zamble, D. B. (2009). Nickel homeostasis and nickel regulation: an overview. Chem Rev 109(10): 4617-4643.
  6. Li, Y. and Zamble, D. B. (2009). pH-responsive DNA-binding activity of Helicobacter pylori NikR. Biochemistry 48(11): 2486-2496.
5 Protocols reviewed
Determination of Recombinant Mannitol-1-phosphatase Activity from Ectocarpus sp.
Authors:  Agnès Groisillier and Thierry Tonon, date: 08/20/2016, view: 4200, Q&A: 0
Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary ...
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Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae
Authors:  David Canadell, Samuel Bru, Josep Clotet and Joaquín Ariño, date: 07/20/2016, view: 5859, Q&A: 0
Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond ...
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25 Protocols edited
Quantitative Analysis of Cargo Density in Single-extracellular Vesicles by Imaging
Authors:  Taketoshi Kajimoto and Shun-ichi Nakamura, date: 12/20/2018, view: 750, Q&A: 0
Function of extracellular vesicles such as exosomes and microvesicles is determined by their wide ranges of cargoes inside them. Even in the pure exosomes or microvesicles the cargo contents are very heterogeneous. To understand this heterogeneous ...
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γ-Secretase Epsilon-cleavage Assay
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are ...
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