Takashi Okamoto Department of Biological Sciences, Tokyo Metropolitan University
3 protocols

Yukinosuke Ohnishi Tokyo Metropolitan University
2 protocols

Erika Toda
  • Biological Sciences, Tokyo Metropolitan University, Japan
Research focus
  • Plant science
  • 2 Author merit
2 Protocols published
Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
Authors:  Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 1068, Q&A: 0
Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered ...
Electro-fusion of Gametes and Subsequent Culture of Zygotes in Rice
Authors:  Erika Toda, Yukinosuke Ohnishi and Takashi Okamoto, date: 12/20/2016, view: 7879, Q&A: 0
Electro-fusion system with isolated gametes has been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, since the female gametophytes of plants are deeply embedded ...
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