Clifford Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
46 protocols

Anita Umesh Illumina
4 protocols

Antoine de Morree Stanford university
34 protocols

Editor
Meenal Sinha
  • Research scientist, University of California San Francisco
Research focus
  • Immunology
  • 1 Author merit

Education

Ph.D., University of Texas at Houston, 2008

1 Protocol published
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:  Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 15161, Q&A: 0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
18 Protocols reviewed
Induced Germinal Center B Cell Culture System
Authors:  Kei Haniuda and Daisuke Kitamura, date: 02/20/2019, view: 4072, Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
Authors:  Michael S. Schappe and Bimal N. Desai, date: 07/20/2018, view: 4488, Q&A: 0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan et ...
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8 Protocols edited
Characterization of Immunological Niches within Peyer’s Patches by ex vivo Photoactivation and Flow Cytometry Analysis
Authors:  Adi Biram and Ziv Shulman, date: 03/20/2020, view: 212, Q&A: 0
T follicular helper (Tfh) cells regulate B cell selection for entry into the germinal center (GC) reaction or for differentiation into antibody forming cells. This process takes place at the border between the T and B zones in lymphoid organs and ...
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Skin Transplantation and Lymphoid Organ Analysis in Mice
Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft ...
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