Clifford Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Andrea Puhar The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Sweden
48 protocols

Anita Umesh Illumina
4 protocols

Antoine de Morree Stanford university
36 protocols

Editor
Meenal Sinha
  • Research scientist, University of California San Francisco
Research focus
  • Immunology
  • 1 Author merit

Education

Ph.D., University of Texas at Houston, 2008

1 Protocol published
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:  Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 17338, Q&A: 1
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
18 Protocols reviewed
Induced Germinal Center B Cell Culture System
Authors:  Kei Haniuda and Daisuke Kitamura, date: 02/20/2019, view: 6388, Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
Authors:  Michael S. Schappe and Bimal N. Desai, date: 07/20/2018, view: 5491, Q&A: 0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan et ...
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11 Protocols edited
Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
Authors:  Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks, date: 05/20/2020, view: 1577, Q&A: 0
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing ...
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Evaluation of B Cell Proliferation in vivo by EdU Incorporation Assay
Authors:  Adi Biram and Ziv Shulman, date: 05/05/2020, view: 1233, Q&A: 0
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens ...
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