Clifford Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
44 protocols

Antoine de Morree Stanford university
33 protocols

Benoit Chassaing Georgia State University
14 protocols

Editor
Meenal Sinha
  • Research scientist, University of California San Francisco
Research focus
  • Immunology
  • 1 Author merit

Education

Ph.D., University of Texas at Houston, 2008

1 Protocol published
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:  Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 13571, Q&A: 0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
18 Protocols reviewed
Induced Germinal Center B Cell Culture System
Authors:  Kei Haniuda and Daisuke Kitamura, date: 02/20/2019, view: 2764, Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
Authors:  Michael S. Schappe and Bimal N. Desai, date: 07/20/2018, view: 3557, Q&A: 0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan et ...
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6 Protocols edited
Adoptive Transfer of Basophils Enriched from Mouse Spleen
Authors:  Adrian M. Piliponsky and Stephen J. Galli, date: 11/05/2019, view: 66, Q&A: 0
CD49b is a member of the integrin family, expressed on basophils, natural killer (NK) cells and a subset CD4+ T cells in the spleen. This protocol describes the adoptive transfer of basophil-enriched CD49b+ cells obtained from ...
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Platelet Isolation and Activation Assays
Authors:  Laura C. Burzynski, Nicholas Pugh and Murray C.H. Clarke, date: 10/20/2019, view: 473, Q&A: 0
Platelets regulate hemostasis and are the key determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets circulate in an inactive state, but become activated in response to damage to the endothelium, which exposes ...
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