Shinji Kasahara Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, USA
1 protocol

Anupam Jhingran Stony Brook University
1 protocol

Tobias M Hohl
  • Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, USA
Research focus
  • Immunology
  • 1 Author merit


Ph.D. in Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, NY, 2001

Current position

Assistant Professor, Department of Medicine, Weill Cornell Medical College, New York, NY
Chief, Infectious Diseases Service, Memorial Sloan Kettering Cancer Center, New York, NY


  1. Jhingran, A., Kasahara, S., Shepardson, K. M., Junecko, B. A., Heung, L. J., Kumasaka, D. K., Knoblaugh, S. E., Lin, X., Kazmierczak, B. I., Reinhart, T. A., Cramer, R. A. and Hohl, T. M. (2015). Heung, L. J. and Hohl, T. M. (2016). DAP12 Inhibits Pulmonary Immune Responses to Cryptococcus neoformans. Infect Immun 84(6): 1879-1886.

  2. Kasahara, S., Jhingran, A., Dhingra, S., Salem, A., Cramer, R. A. and Hohl, T. M. (2016). Role of Granulocyte-Macrophage Colony-Stimulating Factor Signaling in Regulating Neutrophil Antifungal Activity and the Oxidative Burst During Respiratory Fungal Challenge. J Infect Dis 213(8): 1289-1298.

  3. Drummond, R. A., Collar, A. L., Swamydas, M., Rodriguez, C. A., Lim, J. K., Mendez, L. M., Fink, D. L., Hsu, A. P., Zhai, B., Karauzum, H., Mikelis, C. M., Rose, S. R., Ferre, E. M., Yockey, L., Lemberg, K., Kuehn, H. S., Rosenzweig, S. D., Lin, X., Chittiboina, P., Datta, S. K., Belhorn, T. H., Weimer, E. T., Hernandez, M. L., Hohl, T. M., Kuhns, D. B. and Lionakis, M. S. (2015). CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS Pathog 11(12): e1005293.

  4. Clark, H. L., Jhingran, A., Sun, Y., Vareechon, C., de Jesus Carrion, S., Skaar, E. P., Chazin, W. J., Calera, J. A., Hohl, T. M. and Pearlman, E. (2016). Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection. J Immunol 196(1): 336-344.

  5. Panea, C., Farkas, A. M., Goto, Y., Abdollahi-Roodsaz, S., Lee, C., Koscso, B., Gowda, K., Hohl, T. M., Bogunovic, M. and Ivanov, II (2015). Intestinal Monocyte-Derived Macrophages Control Commensal-Specific Th17 Responses. Cell Rep 12(8): 1314-1324.

  6. Jhingran, A., Kasahara, S., Shepardson, K. M., Junecko, B. A., Heung, L. J., Kumasaka, D. K., Knoblaugh, S. E., Lin, X., Kazmierczak, B. I., Reinhart, T. A., Cramer, R. A. and Hohl, T. M. (2015). Compartment-specific and sequential role of MyD88 and CARD9 in chemokine induction and innate defense during respiratory fungal infection. PLoS Pathog 11(1): e1004589.

  7. Shepardson, K. M., Jhingran, A., Caffrey, A., Obar, J. J., Suratt, B. T., Berwin, B. L., Hohl, T. M. and Cramer, R. A. (2014). Myeloid derived hypoxia inducible factor 1-alpha is required for protection against pulmonary Aspergillus fumigatus infection. PLoS Pathog 10(9): e1004378.

  8. Giordano, D., Draves, K. E., Li, C., Hohl, T. M. and Clark, E. A. (2014). Nitric oxide regulates BAFF expression and T cell-independent antibody responses. J Immunol 193(3): 1110-1120.

  9. Fisher, C. E., Stevens, A. M., Leisenring, W., Pergam, S. A., Boeckh, M. and Hohl, T. M. (2014). Independent contribution of bronchoalveolar lavage and serum galactomannan in the diagnosis of invasive pulmonary aspergillosis. Transpl Infect Dis 16(3): 505-510.

  10. Espinosa, V., Jhingran, A., Dutta, O., Kasahara, S., Donnelly, R., Du, P., Rosenfeld, J., Leiner, I., Chen, C. C., Ron, Y., Hohl, T. M. and Rivera, A. (2014). Inflammatory monocytes orchestrate innate antifungal immunity in the lung. PLoS Pathog 10(2): e1003940.

  11. Ngo, L. Y., Kasahara, S., Kumasaka, D. K., Knoblaugh, S. E., Jhingran, A. and Hohl, T. M. (2014). Inflammatory monocytes mediate early and organ-specific innate defense during systemic candidiasis. J Infect Dis 209(1): 109-119.

  12. Kawakami, T., Lichtnekert, J., Thompson, L. J., Karna, P., Bouabe, H., Hohl, T. M., Heinecke, J. W., Ziegler, S. F., Nelson, P. J. and Duffield, J. S. (2013). Resident renal mononuclear phagocytes comprise five discrete populations with distinct phenotypes and functions. J Immunol 191(6): 3358-3372.

  13. Fisher, C. E., Stevens, A. M., Leisenring, W., Pergam, S. A., Boeckh, M. and Hohl, T. M. (2013). The serum galactomannan index predicts mortality in hematopoietic stem cell transplant recipients with invasive aspergillosis. Clin Infect Dis 57(7): 1001-1004.

  14. Grimm, M. J., Vethanayagam, R. R., Almyroudis, N. G., Dennis, C. G., Khan, A. N., D'Auria, A. C., Singel, K. L., Davidson, B. A., Knight, P. R., Blackwell, T. S., Hohl, T. M., Mansour, M. K., Vyas, J. M., Rohm, M., Urban, C. F., Kelkka, T., Holmdahl, R. and Segal, B. H. (2013). Monocyte- and macrophage-targeted NADPH oxidase mediates antifungal host defense and regulation of acute inflammation in mice. J Immunol 190(8): 4175-4184.

  15. Shepardson, K. M., Ngo, L. Y., Aimanianda, V., Latge, J. P., Barker, B. M., Blosser, S. J., Iwakura, Y., Hohl, T. M. and Cramer, R. A. (2013). Hypoxia enhances innate immune activation to Aspergillus fumigatus through cell wall modulation. Microbes Infect 15(4): 259-269.

  16. Ryder, M., Gild, M., Hohl, T. M., Pamer, E., Knauf, J., Ghossein, R., Joyce, J. A. and Fagin, J. A. (2013). Genetic and pharmacological targeting of CSF-1/CSF-1R inhibits tumor-associated macrophages and impairs BRAF-induced thyroid cancer progression. PLoS One 8(1): e54302.

  17. Jhingran, A., Mar, K. B., Kumasaka, D. K., Knoblaugh, S. E., Ngo, L. Y., Segal, B. H., Iwakura, Y., Lowell, C. A., Hamerman, J. A., Lin, X. and Hohl, T. M. (2012). Tracing conidial fate and measuring host cell antifungal activity using a reporter of microbial viability in the lung. Cell Rep 2(6): 1762-1773.

  18. Kinnebrew, M. A., Buffie, C. G., Diehl, G. E., Zenewicz, L. A., Leiner, I., Hohl, T. M., Flavell, R. A., Littman, D. R. and Pamer, E. G. (2012). Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense. Immunity 36(2): 276-287.

  19. Lesokhin, A. M., Hohl, T. M., Kitano, S., Cortez, C., Hirschhorn-Cymerman, D., Avogadri, F., Rizzuto, G. A., Lazarus, J. J., Pamer, E. G., Houghton, A. N., Merghoub, T. and Wolchok, J. D. (2012). Monocytic CCR2(+) myeloid-derived suppressor cells promote immune escape by limiting activated CD8 T-cell infiltration into the tumor microenvironment. Cancer Res 72(4): 876-886.

  20. Shi, C., Hohl, T. M., Leiner, I., Equinda, M. J., Fan, X. and Pamer, E. G. (2011). Ly6G+ neutrophils are dispensable for defense against systemic Listeria monocytogenes infection. J Immunol 187(10): 5293-5298.

  21. Grahl, N., Puttikamonkul, S., Macdonald, J. M., Gamcsik, M. P., Ngo, L. Y., Hohl, T. M. and Cramer, R. A. (2011). In vivo hypoxia and a fungal alcohol dehydrogenase influence the pathogenesis of invasive pulmonary aspergillosis. PLoS Pathog 7(7): e1002145.

  22. Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T. M., Serbina, N. V., Lipuma, L., Leiner, I., Li, M. O., Frenette, P. S. and Pamer, E. G. (2011). Bone marrow mesenchymal stem and progenitor cells induce monocyte emigration in response to circulating toll-like receptor ligands. Immunity 34(4): 590-601.

  23. Rivera, A., Hohl, T. M., Collins, N., Leiner, I., Gallegos, A., Saijo, S., Coward, J. W., Iwakura, Y. and Pamer, E. G. (2011). Dectin-1 diversifies Aspergillus fumigatus-specific T cell responses by inhibiting T helper type 1 CD4 T cell differentiation. J Exp Med 208(2): 369-381.

  24. Ibrahim-Granet, O., Jouvion, G., Hohl, T. M., Droin-Bergere, S., Philippart, F., Kim, O. Y., Adib-Conquy, M., Schwendener, R., Cavaillon, J. M. and Brock, M. (2010). In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis. BMC Microbiol 10: 105.

  25. Shi, C., Velazquez, P., Hohl, T. M., Leiner, I., Dustin, M. L. and Pamer, E. G. (2010). Monocyte trafficking to hepatic sites of bacterial infection is chemokine independent and directed by focal intercellular adhesion molecule-1 expression. J Immunol 184(11): 6266-6274.

  26. Mircescu, M. M., Lipuma, L., van Rooijen, N., Pamer, E. G. and Hohl, T. M. (2009). Essential role for neutrophils but not alveolar macrophages at early time points following Aspergillus fumigatus infection. J Infect Dis 200(4): 647-656.

  27. Serbina, N. V., Hohl, T. M., Cherny, M. and Pamer, E. G. (2009). Selective expansion of the monocytic lineage directed by bacterial infection. J Immunol 183(3): 1900-1910.

  28. Hohl, T. M., Rivera, A., Lipuma, L., Gallegos, A., Shi, C., Mack, M. and Pamer, E. G. (2009). Inflammatory monocytes facilitate adaptive CD4 T cell responses during respiratory fungal infection. Cell Host Microbe 6(5): 470-481.

  29. Hohl, T. M., Feldmesser, M., Perlin, D. S. and Pamer, E. G. (2008). Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure. J Infect Dis 198(2): 176-185.

  30. Rivera, A., Van Epps, H. L., Hohl, T. M., Rizzuto, G. and Pamer, E. G. (2005). Distinct CD4+-T-cell responses to live and heat-inactivated Aspergillus fumigatus conidia. Infect Immun 73(11): 7170-7179.

  31. Hohl, T. M., Van Epps, H. L., Rivera, A., Morgan, L. A., Chen, P. L., Feldmesser, M. and Pamer, E. G. (2005). Aspergillus fumigatus triggers inflammatory responses by stage-specific beta-glucan display. PLoS Pathog 1(3): e30.

  32. Roorda, R. D., Hohl, T. M., Toledo-Crow, R. and Miesenbock, G. (2004). Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes. J Neurophysiol 92(1): 609-621.

  33. Wimmer, C., Hohl, T. M., Hughes, C. A., Muller, S. A., Sollner, T. H., Engel, A. and Rothman, J. E. (2001). Molecular mass, stoichiometry, and assembly of 20 S particles. J Biol Chem 276(31): 29091-29097.

  34. Fleming, K. G., Hohl, T. M., Yu, R. C., Muller, S. A., Wolpensinger, B., Engel, A., Engelhardt, H., Brunger, A. T., Sollner, T. H. and Hanson, P. I. (1998). A revised model for the oligomeric state of the N-ethylmaleimide-sensitive fusion protein, NSF. J Biol Chem 273(25): 15675-15681.

  35. Hohl, T. M., Parlati, F., Wimmer, C., Rothman, J. E., Sollner, T. H. and Engelhardt, H. (1998). Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes. Mol Cell 2(5): 539-548.

  36. Baldini, G., Hohl, T., Lin, H. Y. and Lodish, H. F. (1992). Cloning of a Rab3 isotype predominantly expressed in adipocytes. Proc Natl Acad Sci U S A 89(11): 5049-5052.

1 Protocol published
Flow Cytometry of Lung and Bronchoalveolar Lavage Fluid Cells from Mice Challenged with Fluorescent Aspergillus Reporter (FLARE) Conidia
Authors:  Anupam Jhingran, Shinji Kasahara and Tobias M Hohl, date: 09/20/2016, view: 11699, Q&A: 0
Aspergillus fumigatus is a ubiquitous fungal pathogen that forms airborne conidia. The process of restricting conidial germination into hyphae by lung leukocytes is critical in determining infectious outcomes. Tracking the outcome of ...
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