Ken Shirasu Center for Sustainable Resource Science, RIKEN
3 protocols

Jonathan Jones The Sainsbury Laboratory, Norwich Research Park, UK
1 protocol

Shuta Asai
  • Center for Sustainable Resource Science, RIKEN
Research focus
  • Plant science
  • 1 Author merit


Ph.D. in Agricultural Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Japan, 2009

Current position

Postdoctoral Researcher, Center for Sustainable Resource Science, RIKEN, Japan


  1. Asai, S. and Shirasu, K. (2015). Plant cells under siege: plant immune system versus pathogen effectors. Curr Opin Plant Biol 28: 1-8.
  2. Asai, S., Rallapalli, G., Piquerez, S. J., Caillaud, M. C., Furzer, O. J., Ishaque, N., Wirthmueller, L., Fabro, G., Shirasu, K. and Jones, J. D. (2014). Expression profiling during arabidopsis/downy mildew interaction reveals a highly-expressed effector that attenuates responses to salicylic acid. PLoS Pathog 10(10): e1004443.
  3. Wirthmueller, L., Roth, C., Fabro, G., Caillaud, M. C., Rallapalli, G., Asai, S., Sklenar, J., Jones, A. M., Wiermer, M., Jones, J. D. and Banfield, M. J. (2015). Probing formation of cargo/importin-alpha transport complexes in plant cells using a pathogen effector. Plant J 81(1): 40-52.
  4. Kadota, Y., Sklenar, J., Derbyshire, P., Stransfeld, L., Asai, S., Ntoukakis, V., Jones, J. D., Shirasu, K., Menke, F., Jones, A. and Zipfel, C. (2014). Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. Mol Cell 54(1): 43-55.
  5. Caillaud, M. C., Asai, S., Rallapalli, G., Piquerez, S., Fabro, G. and Jones, J. D. (2013). A downy mildew effector attenuates salicylic acid-triggered immunity in Arabidopsis by interacting with the host mediator complex. PLoS Biol 11(12): e1001732.
  6. Asai, S., Ichikawa, T., Nomura, H., Kobayashi, M., Kamiyoshihara, Y., Mori, H., Kadota, Y., Zipfel, C., Jones, J. D. and Yoshioka, H. (2013). The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. J Biol Chem 288(20): 14332-14340.
  7. Bellin D., Asai S., Delledonne M. and Yoshioka H., “Nitric oxide as a mediator for defense responses” Molecular Plant-Microbe Interactions 26:271-277, 2013.
  8. Caillaud, M. C., Wirthmueller, L., Fabro, G., Piquerez, S. J., Asai, S., Ishaque, N. and Jones, J. D. (2012). Mechanisms of nuclear suppression of host immunity by effectors from the Arabidopsis downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa). Cold Spring Harb Symp Quant Biol 77: 285-293.
  9. Kobayashi, M., Yoshioka, M., Asai, S., Nomura, H., Kuchimura, K., Mori, H., Doke, N. and Yoshioka, H. (2012). StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. New Phytol 196(1): 223-237.
  10. Asai, S., Yoshioka, M., Nomura, H., Tone, C., Nakajima, K., Nakane, E., Doke, N. and Yoshioka, H. (2011). A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production. J Gen Plant Pathol 77:152-162.
  11. Yoshioka, H., Mase, K., Yoshioka, M., Kobayashi, M. and Asai, S. (2011). Regulatory mechanisms of nitric oxide and reactive oxygen species generation and their role in plant immunity. Nitric Oxide 25(2): 216-221.
  12. Asai, S., Mase, K. and Yoshioka, H. (2010). A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance. Plant J 62(6): 911-924.
  13. Asai, S., Mase, K. and Yoshioka, H. (2010). Role of nitric oxide and reactive oxygen [corrected] species in disease resistance to necrotrophic pathogens. Plant Signal Behav 5(7): 872-874.
  14. Asai, S. and Yoshioka, H. (2009). Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrytis cinerea in Nicotiana benthamiana. Mol Plant Microbe In 22:619-629.
  15. Yoshioka, H., Asai, S., Yoshioka, M. and Kobayashi, M. (2009). Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity. Mol Cells 28(4): 321-329.
  16. Asai, S., Ohta, K. and Yoshioka, H. (2006). MAPKs signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Plant Cell 20:1390-1406.
  17. Asai, S. and Yoshioka, H. (2008). The role of radical burst via MAPK signaling in plant immunity. Plant Signal Behav 3(11): 920-922.
  18. Kato, H., Asai, S., Yamamoto-Katou, A., Yoshioka, H., Doke, N. and Kawakita, K. (2007). Involvement of NbNOA1 in NO production and defense in INF1-treated Nicotiana benthamiana. J Gen Plant Pathol 74:15-23.
  19. Kudo, C., Suzuki, T., Fukuoka, S., Asai, S., Suenaga, H., Sasabe, M., Takano, Y., Okuno, T., Toyoda, K., Shiraishi, T., Ichinose, Y. and Inagaki, Y. S. (2007). Suppression of Cdc27B expression induces plant defence responses. Mol Plant Pathol 8(4): 365-373.
1 Protocol published
Hyaloperonospora arabidopsidis (Downy Mildew) Infection Assay in Arabidopsis
Authors:  Shuta Asai, Ken Shirasu and Jonathan D. G. Jones, date: 10/20/2015, view: 12089, Q&A: 2
Hyaloperonospora arabidopsidis (Hpa; formerly Peronospora parasitica or Hyaloperonospora parasitica) is an oomycete downy mildew pathogen of the model plant Arabidopsis. The pathosystem between ...
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