Maria D’Império-Lima Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil
3 protocols

Elena Lassunskaia Laboratório de Biologia do Reconhecer, Universidade Estadual do Norte Fluminense (UENF), Brazil
1 protocol

Eduardo P. Amaral
  • Departamento de Imunologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), Brazil
Research focus
  • Immunology
  • 1 Author merit


M.S. in Immunology, Department of Immunology, University of São Paulo, Brazil, 2011

Current position

Ph.D. student in Immunology, Department of Immunology, University of São Paulo, Brazil


  1. Amaral, E. P., Ribeiro, S. C., Lanes, V. R., Almeida, F. M., de Andrade, M. R., Bomfim, C. C., Salles, E. M., Bortoluci, K. R., Coutinho-Silva, R., Hirata, M. H., Alvarez, J. M., Lasunskaia, E. B. and D'Imperio-Lima, M. R. (2014). Pulmonary infection with hypervirulent Mycobacteria reveals a crucial role for the P2X7 receptor in aggressive forms of tuberculosis. PLoS Pathog 10(7): e1004188.
  2.  Mayer-Barber, K. D., Andrade, B. B., Oland, S. D., Amaral, E. P., Barber, D. L., Gonzales, J., Derrick, S. C., Shi, R., Kumar, N. P., Wei, W., Yuan, X., Zhang, G., Cai, Y., Babu, S., Catalfamo, M., Salazar, A. M., Via, L. E., Barry, C. E., 3rd and Sher, A. (2014). Host-directed therapy of tuberculosis based on interleukin-1 and type I interferon crosstalk. Nature 511(7507): 99-103.
  3. Ribeiro, S. C., Gomes, L. L., Amaral, E. P., Andrade, M. R., Almeida, F. M., Rezende, A. L., Lanes, V. R., Carvalho, E. C., Suffys, P. N., Mokrousov, I. and Lasunskaia, E. B. (2014). Mycobacterium tuberculosis strains of the modern sublineage of the Beijing family are more likely to display increased virulence than strains of the ancient sublineage. J Clin Microbiol 52(7): 2615-2624.
  4. da Silva, H. B., Amaral, E. P., Nolasco, E. L., de Victo, N. C., Atique, R., Jank, C. C., Anschau, V., Zerbini, L. F. and Correa, R. G. (2013). Dissecting major signaling pathways throughout the development of prostate cancer. Prostate Cancer 2013: 920612.
  5. Lage, S. L., Buzzo, C. L., Amaral, E. P., Matteucci, K. C., Massis, L. M., Icimoto, M. Y., Carmona, A. K., D'Imperio Lima, M. R., Rodrigues, M. M., Ferreira, L. C., Amarante-Mendes, G. P. and Bortoluci, K. R. (2013). Cytosolic flagellin-induced lysosomal pathway regulates inflammasome-dependent and -independent macrophage responses. Proc Natl Acad Sci U S A 110(35): E3321-3330.
  6. Andrade, M. R., Amaral, E. P., Ribeiro, S. C., Almeida, F. M., Peres, T. V., Lanes, V., D'Imperio-Lima, M. R. and Lasunskaia, E. B. (2012). Pathogenic Mycobacterium bovis strains differ in their ability to modulate the proinflammatory activation phenotype of macrophages. BMC Microbiol 12: 166.
  7. Amaral, E. P., Kipnis, T. L., de Carvalho, E. C., da Silva, W. D., Leao, S. C. and Lasunskaia, E. B. (2011). Difference in virulence of Mycobacterium avium isolates sharing indistinguishable DNA fingerprint determined in murine model of lung infection. PLoS One 6(6): e21673.
  8. Martins de Sousa, E., Bonfim de Bortoli, F., Amaral, E. P., Batista, A. C., Liberman Kipnis, T., Marques Cardoso, A., Kipnis, A. and Junqueira-Kipnis, A. P. (2010). Acute immune response to Mycobacterium massiliense in C57BL/6 and BALB/c mice. Infect Immun 78(4): 1571-1581.
1 Protocol published
Isolation of Lung Infiltrating Cell in Mice
Authors:  Eduardo P. Amaral, Elena B. Lassunskaia and Maria Regina D’Império-Lima, date: 02/20/2015, view: 16787, Q&A: 0
Inflammatory lung diseases induce strong leukocyte recruitment into the organ, culminating in pneumonia area formation. Here, we describe the protocol for isolation of lung infiltrating cells. Using this assay, we analyzed the lung cell phenotyping ...
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