Vivien Coulson-Thomas University of Houston
4 protocols
Vivien Coulson-Thomas University of Houston
22 protocols

tarsis ferreira
  • Biotech engineer, OPTIMVIA
Research focus
  • Biochemistry
  • glycosaminoglycans
  • 1 Author merit

Education

PhD

Lab information

PI

1 Protocol published
Dimethylmethylene Blue Assay (DMMB)
Authors:  Vivien Jane Coulson- Thomas and Tarsis Ferreira Gesteira, date: 09/20/2014, view: 43171, Q&A: 8
Glycosaminoglycans (GAGs) are long unbranched polysaccharides consisting of repeating disaccharide units composed of a hexosamine (glucosamine or galactosamine) and a hexuronic acid (glucuronic or iduronic acid). Depending on the disaccharide unit ...
3 Protocols reviewed
A Robust, One-step FRET Assay for Human Heparanase
Authors:  Jyothi C. Sistla and Umesh R. Desai, date: 09/05/2019, view: 1125, Q&A: 0
Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of ...
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Muscle Function Assessment Using a Drosophila Larvae Crawling Assay
Authors:  Yanina Post and Achim Paululat, date: 07/20/2018, view: 3078, Q&A: 0
Here we describe a simple method to measure larval muscle contraction and locomotion behavior. The method enables the user to acquire data, without the necessity of using expensive equipment (Rotstein et al., 2018). To measure contraction ...
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