Frances Separovic The University of Melbourne
2 protocols

Neil O’Brien-Simpson Melbourne Dental School, The University of Melbourne, Australia
2 protocols

Eric Reynolds Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Katrina Walsh Melbourne Dental School, The University of Melbourne, Australia
1 protocol
Abhilash Padavannil University of California Davis
1 protocol

Agnieszka Zienkiewicz Nicolaus Copernicus University
23 protocols

Alka Mehra Institute of Genomics and Integrative Biology
41 protocols

Anca Savulescu University of Cape Town
23 protocols

Editor
Marc-Antoine Sani
  • Research Fellow, University of Melbourne
Research focus
  • Biophysics
  • Structural biology, NMR spectroscopy, lipid-protein interactions, biophysics
  • 2 Author merit

Education

PhD, University of Bordeaux / Umea University, 2008

Lab information

Group leader: Frances Separovic

Publications

2 Protocols published
Bacterial Fluorescent-dextran Diffusion Assay
Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance ...
Dye Release Experiments with Dextran Loaded Vesicles
Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 8475, Q&A: 0
Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously ...
6 Protocols reviewed
Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
Authors:  Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu, date: 02/20/2021, view: 2389, Q&A: 0

Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules

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Measurement of Protein-Protein Interactions through Microscale Thermophoresis (MST)
Authors:  Magnez Romain, Bryan Thiroux, Morgane Tardy, Bruno Quesnel and Xavier Thuru, date: 04/05/2020, view: 3220, Q&A: 0
The binding interactions of PD-1 and PD-L1 have been studied by surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) over the past few years, but these investigations resulted in controversy regarding the values of the ...
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9 Protocols edited
Structural and Functional Mapping of Mesenchymal Bodies

The formation of spheroids with mesenchymal stem/stromal cells (MSCs), mesenchymal bodies (MBs), is usually performed using bioreactors or conventional well plates. While these methods promote the formation of a large number of spheroids, they

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Using Atomic Force Microscopy to Study the Real Time Dynamics of DNA Unwinding by Mitochondrial Twinkle Helicase
Authors:  Parminder Kaur, Hai Pan, Matthew J. Longley, William C. Copeland and Hong Wang, date: 09/05/2021, view: 912, Q&A: 0

Understanding the structure and dynamics of DNA-protein interactions during DNA replication is crucial for elucidating the origins of disorders arising from its dysfunction. In this study, we employed Atomic Force Microscopy as a single-molecule

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