Alka Mehra Institute of Genomics and Integrative Biology
21 protocols

Andrea Introini Karolinska Institutet
8 protocols

Balaji Olety Amaranath University of Massachusetts medical School
2 protocols

Dennis Nürnberg Imperial College London
42 protocols

Reviewer
Kathrin Sutter
  • University of Duisburg-Essen
Research focus
  • Immunology
  • 2 Author merit
2 Protocols published
Measurement of IFN-α Subtype Concentrations (Virus-free, Cell-based Bioassay)
Author:  Kathrin Gibbert, date: 06/20/2013, view: 5152, Q&A: 0
The induction of type I IFN is the immediate host response against viral infections. Type I IFNs belong to a multigene family including up to 14 different IFN-α subtypes and one IFN-β. They are highly conserved and bind the same receptor (IFNAR1/2) ...
IFN-α Inhibition Assay in vitro
Author:  Kathrin Gibbert, date: 06/20/2013, view: 5932, Q&A: 0
During viral infections Interferon-α (IFN-α) is expressed by infected host cells. IFN-α binds to its receptor (IFNAR1/2), which leads to the activation of downstream signaling via JAK-STAT. This signaling cascade results in the expression of several ...
14 Protocols reviewed
Isolation of Splenic Dendritic Cells Using Fluorescence-activated Cell Sorting
Authors:  Simon J Tavernier, Fabiola Osorio, Sophie Janssens and Bart N Lambrecht, date: 03/05/2015, view: 9548, Q&A: 0
The spleen is a vastly vasculated organ and consists of a complex organized network of innate and adaptive immune cells. This permits the specialized functions of the spleen such as antibacterial and antifungal immunity and iron metabolism among ...
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Purification of Herpesvirus Virions and Capsids
Authors:  Xinghong Dai and Z. Hong Zhou, date: 08/05/2014, view: 8559, Q&A: 43
This protocol was designed for large-scale purification of herpesvirus particles by cell culture. Virions and capsids are isolated from extracellular culture media and cell nuclei, respectively. Purity and concentration of the purified samples are ...
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