Ryutaro Utsumi Bioscience Department, Kinki University, Japan
1 protocol

Eiji Ishii Bioscience Department, Kinki University, Japan
1 protocol
Adam Idoine Carnegie Institution
13 protocols

Aksiniya Asenova University of California, Davis
13 protocols

Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
44 protocols

Arsalan Daudi University of California
157 protocols

Reviewer
Yoko Eguchi
  • Faculty, Kindai University
Research focus
  • Microbiology
  • 1 Author merit
1 Protocol published
Protein-peptide Interaction by Surface Plasmon Resonance
Authors:  Eiji Ishii, Yoko Eguchi and Ryutaro Utsumi, date: 02/05/2013, view: 15269, Q&A: 0
This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ...
8 Protocols reviewed
Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for in situ Labeling of Peptidoglycan
Authors:  Katharina Peters, Manuel Pazos, Michael S. VanNieuwenhze and Waldemar Vollmer, date: 08/05/2019, view: 532, Q&A: 0
The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves ...
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Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:  Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich, date: 07/20/2017, view: 3784, Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...
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