AK
Ajay Kumar
  • CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow
Research fields
  • Pharmacology
Microbial Mutagenicity Assay: Ames Test
Authors:  Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar, date: 03/20/2018, view: 32230, Q&A: 2
The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, reagents, cosmetics, waste water, pesticides and other substances which are easily solubilized in a liquid suspension. We present the protocol for conducting Ames test in the laboratory.
Ames Test
[Feedback 1] Dear Sir/Mam I have read your article in bio-protocol and found so many mistakes in the protocol. Please go through the composition of Vogel Bonner Medium Recipe 1 in your protocol. 1) All the constituents listed are in mg (miligram), but actually it is in grams. 2) the composition Agar mentioned in recipe 9, 10 and 11 in mg but actually it is in grams. 3) Please check all the compositions carefully. The Protocol mentioned here is not reproducible because article have so many composition error..
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