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The protocol is structured in an appropriate format that clearly describes the experimental procedure, including separate sections for the abstract, background, materials and reagents, equipment, procedure, data analysis, recipes, acknowledgements, and references (see Protocol Preparation Guidelines for our recommended format).

There is demonstrable evidence that the submitted protocol works (for example, the protocol was previously published in its brief version by the authors as part of a research article).

The protocol contains sufficient detail to enable other researchers to effectively reproduce the steps described.

The protocol is clearly written in formal English.

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The protocol adheres to all relevant standards (e.g. NIH for NIH-funded researchers) for experimentation and research integrity.

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