Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
[Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...
Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
[Abstract] Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca2+-dependent and redox-dependent signals. Conventional in ...
Method for Assessing Virulence of Colletotrichum higginsianum on Arabidopsis thaliana Leaves Using Automated Lesion Area Detection and Measurement
Authors: Ayako Tsushima,
Pamela Gan and
Ken Shirasu,
date: 11/20/2019,
view: 916,
Q&A: 0
[Abstract] The plant pathogenic fungus, Colletotrichum higginsianum is widely used to understand infection mechanisms, as it infects the model plant Arabidopsis thaliana. To determine the virulence of C. higginsianum, several methods have been developed, such as disease reaction scoring, lesion measurement, entry rate assays, and ...
Cell Wall Compositional Analysis of Rice Culms
Authors: Lanjun Zhang,
Baocai Zhang and
Yihua Zhou,
date: 10/20/2019,
view: 546,
Q&A: 0
[Abstract] The plant cell wall is a complicated network that is mainly constituted of polysaccharides, such as cellulose, hemicellulose and pectin. Many noncellulosic polysaccharides are further acetylated, which confers these polymers flexible physicochemical properties. Due to the significance of cell wall in plant growth and development, the analytic ...
PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
[Abstract] The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of ...