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Confocal Microscopy of Reovirus Transport in Living Dorsal Root Ganglion Neurons

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Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue

Authors: André Kuhn and Lars Østergaard, date: 12/05/2020, view: 22, Q&A: 0

Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone ...

Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry

Authors: Keshun Yu, Huazhen Liu and Pradeep Kachroo, date: 12/05/2020, view: 28, Q&A: 0

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Chromatin Immunoprecipitation (ChIP) to Assess Histone Marks in Auxin-treated Arabidopsis thaliana Inflorescence Tissue

Authors: André Kuhn and Lars Østergaard, date: 12/05/2020, view: 22, Q&A: 0

[Abstract] Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) or high-throughput sequencing (ChIP-seq) has become the gold standard for the identification of binding sites of DNA binding proteins and the localization of histone modification on a locus-specific or genome-wide scale, respectively. ChIP experiments can be divided into seven ...

Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry

Authors: Keshun Yu, Huazhen Liu and Pradeep Kachroo, date: 12/05/2020, view: 28, Q&A: 0

[Abstract]

Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

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Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum)

Authors: Natasha Navet and Miaoying Tian, date: 11/20/2020, view: 643, Q&A: 1

[Abstract]

Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding and deploy effective disease management strategies, and breeding through genetic engineering. Here we

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K⁺ Release Assay and K⁺ Measurement in Oocyte Assay

Author: Hong Li, date: 11/05/2020, view: 574, Q&A: 0

[Abstract] The Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a number of potassium transporters and channels have been identified in oocytes expression system by the two-electrode voltage clamp technology. It is difficult to characterize K⁺ ...

Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages

Authors: Jill Kowal, Elena Arrigoni and Sophie Lane, date: 10/20/2020, view: 364, Q&A: 0

[Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...

Low-cost and High-throughput RNA-seq Library Preparation for Illumina Sequencing from Plant Tissue

Authors: Marta Bjornson, Kaisa Kajala, Cyril Zipfel and Pingtao Ding, date: 10/20/2020, view: 744, Q&A: 0

[Abstract] Transcriptome analysis can provide clues to biological processes affected in different genetic backgrounds or/and under various conditions. The price of RNA sequencing (RNA-seq) has decreased enough so that medium- to large-scale transcriptome analyses in a range of conditions are feasible. However, the price and variety of options for library ...

Quantification of Methylglyoxal Levels in Cowpea Leaves in Response to Cowpea Aphid Infestation

Authors: Jacob R. MacWilliams, Monika Ostaszewska-Bugajska, Klaudia Borysiuk, Bożena Szal and Isgouhi Kaloshian, date: 10/20/2020, view: 272, Q&A: 0

[Abstract] Aphids are a serious pest of crops across the world. Aphids feed by inserting their flexible hypodermal needlelike mouthparts, or stylets, into their host plant tissues. They navigate their way to the phloem where they feed on its sap causing little mechanical damage to the plant. Additionally, while feeding, aphids secrete proteinaceous effectors ...

Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis

Authors: Yu Jin and Sebastian Marquardt, date: 10/20/2020, view: 594, Q&A: 0

[Abstract] CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles that offer a straightforward starting point for functional dissections of genomic loci. We present an ...

Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides

Authors: Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle, date: 10/05/2020, view: 874, Q&A: 0

[Abstract] The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and oriented tissue growth by tuning its local mechano-chemical properties. To refine our knowledge of these ...

A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips

Author: Nguyen Hoai Nguyen, date: 10/05/2020, view: 600, Q&A: 0

[Abstract] Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...

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