PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
[Abstract] The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of ...
Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
[Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
Measuring Protein Half-life in Arabidopsis thaliana
Authors: Hongqing Guo and
Yanhai Yin,
date: 08/05/2019,
view: 326,
Q&A: 0
[Abstract] Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is a translational inhibitor that inhibits protein synthesis via cytoplasmic ribosomes. Here we ...
Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
[Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
Transient Expression Assay in Strawberry Fruits
Authors: Mengting Pi,
Qi Gao and
Chunying Kang,
date: 06/05/2019,
view: 914,
Q&A: 0
[Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...