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    Protocols in Current Issue
    Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
    Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its’ inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic ...
    Rapid Screening and Evaluation of Maize Seedling Resistance to Stalk Rot Caused by Fusarium spp.
    Authors:  Yali Sun, Xinsen Ruan, Liang Ma, Fang Wang and Xiquan Gao, date: 05/20/2018, view: 108, Q&A: 0
    Corn stalk rot caused by Fusarium spp., a genus of soil-borne fungal pathogens, has become a major concern of maize production. This disease normally causes significant reduction of maize yield and quality worldwide. The field assay for ...
    Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
    [Abstract] Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its’ inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to ...
    Rapid Screening and Evaluation of Maize Seedling Resistance to Stalk Rot Caused by Fusarium spp.
    Authors:  Yali Sun, Xinsen Ruan, Liang Ma, Fang Wang and Xiquan Gao, date: 05/20/2018, view: 108, Q&A: 0
    [Abstract] Corn stalk rot caused by Fusarium spp., a genus of soil-borne fungal pathogens, has become a major concern of maize production. This disease normally causes significant reduction of maize yield and quality worldwide. The field assay for identifying stalk rot resistance using adult plants is largely relying on large population, yet ...
    Infection Process Observation of Magnaporthe oryzae on Barley Leaves
    Author:  Xiao-Lin Chen, date: 05/05/2018, view: 212, Q&A: 0
    [Abstract] Rice blast and wheat blast caused by Magnaporthe oryzae is a serious threat to rice and wheat production. Appropriate methods for observing M. oryzae infection process are important for study of the fungal infection mechanisms, plant resistance reactions, and host-M. oryzae interactions. The rice leaf sheath is commonly ...
    Evaluation of the Condition of Respiration and Photosynthesis by Measuring Chlorophyll Fluorescence in Cyanobacteria
    Authors:  Takako Ogawa and Kintake Sonoike , date: 05/05/2018, view: 126, Q&A: 0
    [Abstract] Chlorophyll fluorescence measurements have been widely used to monitor the condition of photosynthesis. Furthermore, chlorophyll fluorescence from cyanobacteria reflects the condition of respiration, since cyanobacterial photosynthesis shares several components of electron transport chain with respiration. This protocol presents the method to ...
    Functional Evaluation of the Signal Peptides of Secreted Proteins
    Authors:  Weixiao Yin, Yufu Wang, Tao Chen, Yang Lin and Chaoxi Luo, date: 05/05/2018, view: 149, Q&A: 0
    [Abstract] Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal peptide is transformed into yeast. The function of the signal peptides can be evaluated by using ...
    Detecting the Interaction of Double-stranded RNA Binding Protein, Viral Protein and Primary miRNA Transcript by Co-immunoprecipitation in planta
    Authors:  Lijia Zheng, Chao Zhang, Jianguo Wu and Yi Li, date: 05/05/2018, view: 144, Q&A: 0
    [Abstract] MicroRNAs (miRNAs) play important roles in plant growth, development, and response to infection by microbes. Double-stranded RNA binding protein 1 (DRB1) facilitates the processing of primary miRNA transcripts into mature miRNAs. Recently, we found that NS3 protein encoded by rice stripe virus (RSV) associates with DRB1 and promotes miRNA ...
    In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato
    Authors:  Jiajie Lian, Hongyu Han, Jiuhai Zhao and Chuanyou Li, date: 04/20/2018, view: 321, Q&A: 0
    [Abstract] Botrytis cinerea (B. cinerea) attacks many crops of economic importance, represents one of the most extensively studied necrotrophic pathogens. Inoculation of B. cinerea and phenotypic analysis of plant resistance are key procedures to investigate the mechanism of plant immunity. Here we describe a protocol for B. ...
    Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach
    [Abstract] Lipid peroxidation is a physiological indicator of both biotic and abiotic stress responses, hence is often used as a biomarker to assess stress-induced cell damage or death. Here we demonstrate an easy, quick and cheap staining method to assess lipid peroxidation in plant tissues. In this methodology, Schiff’s reagent, is used to assay for ...
    Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
    Authors:  Isabella G. S. Visschers, Nicole M. van Dam and Janny L. Peters, date: 04/20/2018, view: 395, Q&A: 0
    [Abstract] Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available ...
    In vitro Nitrate Reductase Activity Assay from Arabidopsis Crude Extracts
    Authors:  Joo Yong Kim and Hak Soo Seo, date: 04/05/2018, view: 351, Q&A: 0
    [Abstract] Nitrate reductase (NR) reduces the major plant nitrogen source, NO3-, into NO2-. NR activity can be measured by its final product, nitrite through its absorbance under optimized condition. Here, we present a detailed protocol for measuring relative enzyme activity of NR from Arabidopsis crude ...