Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus
Authors: Haiqin Yao,
Delphine Scornet,
Yacine Badis,
Akira F. Peters,
Murielle Jam,
Cecile Hervé,
Philippe Potin,
Susana M. Coelho and
J. Mark Cock,
date: 09/20/2020,
view: 107,
Q&A: 0
[Abstract] The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
Authors: Van T. Luu,
Melissa Stiebner,
Paula Emmerich Maldonado,
Sandra Valdés,
Didier Marín,
Gerardo Delgado,
Virginia Laluz,
Lin-Bo Wu,
Paul Chavarriaga,
Joe Tohme,
Inez H. Slamet-Loedin and
Wolf B. Frommer,
date: 09/05/2020,
view: 1331,
Q&A: 0
[Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
The ATPase Activity of Escherichia coli Expressed AAA+-ATPase Protein
[Abstract] ATPases are the enzymes that breakdown ATP to ADP and release inorganic phosphate (Pi). Here we provide a detailed protocol to determine the ATPase activity of a recombinant AAA+-ATPase protein (GENERAL CONTROL NON-REPRESSIBLE-4 [GCN4]) by spectrophotometric absorption at 360 nm to measure the accumulated inorganic phosphate. In ...
Generation and in Planta Functional Analysis of Potato Virus Y mutants
Authors: Katja Stare,
Anna Coll,
Ion Gutiérrez-Aguirre,
Magda Tušek Žnidarič,
Maja Ravnikar,
Andreja Kežar,
Luka Kavčič,
Marjetka Podobnik and
Kristina Gruden,
date: 07/20/2020,
view: 415,
Q&A: 0
[Abstract] Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to ...
RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
Authors: Tianran Jia and
Brandon H. Le,
date: 07/20/2020,
view: 391,
Q&A: 0
[Abstract] Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive ...