Hypoxia Reporter Element Assay
Authors: Daelynn R Buelow and
Sharyn D. D Baker,
date: 08/05/2018,
view: 77,
Q&A: 0
[Abstract] Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the ...
Mouse Mammary Gland Whole Mount Preparation and Analysis
Authors: Cornelia Tolg,
Mary Cowman and
Eva A. Turley,
date: 07/05/2018,
view: 451,
Q&A: 0
[Abstract] The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure a method is needed that provides an overview of the entire gland. Whole mounts provide this ...
Isolation of Microvascular Endothelial Cells
[Abstract] The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. Murine endothelial cell culture is an important tool for cardiovascular disease research. This protocol demonstrates a quick, efficient method for the isolation of microvascular endothelial cells from murine ...
3D Culture Protocol for Testing Gene Knockdown Efficiency and Cell Line Derivation
[Abstract] Traditional 2D cell cultures with cells grown as monolayers on solid surface still represent the standard method in cancer research for drug testing. Cells grown in 2D cultures, however, lack relevant cell-matrix and cell-cell interactions and ignore the true three-dimensional anatomy of solid tumors. Cells cultured in 2D can also undergo ...
Hyaluronan Isolation from Mouse Mammary Gland
Authors: Cornelia Tolg,
Mary Cowman and
Eva A. Turley,
date: 06/05/2018,
view: 429,
Q&A: 0
[Abstract] The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is ...
Generation of Luciferase-expressing Tumor Cell Lines
[Abstract] Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. Essential to these studies is the ability to both track tumor development and quantify tumor burden in vivo. For this purpose, the introduction of genes that confer tumors with bioluminescent ...