Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice
[Abstract] Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...
In situ, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses
Authors: Katrin Hufnagel,
Dennis Reininger,
Siu Wang Ng,
Nadine Gassert,
Juliane K. Rohland,
Soroosh Shahryarhesami,
Andrea S. Bauer,
Tim Waterboer and
Jörg D. Hoheisel,
date: 02/05/2019,
view: 454,
Q&A: 0
[Abstract] Until recently, whole-proteome microarrays for comprehensive studies of protein interactions were mostly produced by individual cloning and cellular expression of very many open reading frames, followed by protein isolation and purification as well as array production. To overcome this cumbersome process, we have developed a method to generate ...
Adoptive Transfer of Monocytes Sorted from Bone Marrow
[Abstract] Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to ...
Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
Authors: Selene K. Roberts,
Michael Hirsch,
Alexandra McStea,
Laura C. Zanetti-Domingues,
David T. Clarke,
Jeroen Claus,
Peter J. Parker,
Lin Wang and
Marisa L. Martin-Fernande,
date: 12/05/2018,
view: 714,
Q&A: 0
[Abstract] The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is overexpressed in many tumors, and the deregulated proliferation of cancerous cells is driven by cooperation with ...
In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
[Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.
Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...