Bone-in-culture Array to Model Bone Metastasis in ex vivo Condition
Authors: Hai Wang and
Xiang H.-F. Zhang,
date: 01/20/2020,
view: 110,
Q&A: 0
[Abstract] Bone is the most frequently affected organ by metastases of breast cancer and prostate cancer. Our knowledge on bone metastasis is extremely limited due to the lack of potent and efficient experimental models. We developed the “Bone-In-Culture Array (BICA)” platform to model the bone colonization of cancer cells in ex vivo cultures. The ...
Automated Analysis of Cell Surface Ruffling: Ruffle Quantification Macro
[Abstract] Cell surface protrusions include F-actin rich, wave-like ruffles that are erected transiently in response to stimuli and during cell migration. Macrophages are innate immune cells that ruffle constitutively and more dramatically in cells activated by pathogens. Dorsal ruffles and their resulting macropinosomes are key sites for environmental ...
Analysis of Random Migration of Cancer Cells in 3D
Authors: Sai P. Visweshwaran and
Alexis Gautreau,
date: 01/05/2020,
view: 326,
Q&A: 0
[Abstract] The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D ...
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
[Abstract] Immuno-PCR (IPCR) is a powerful method in antigen detection where a PCR-amplifiable DNA reporter is conjugated to a specific antibody or an aptamer for the target molecule. In the development and application of IPCR, successful conjugation of a protein (an antibody) with a reporter DNA becomes challenging. To address this issue, we recently ...
An Optimized Method to Isolate Human Fibroblasts from Tissue for Ex Vivo Analysis
Authors: Sara Waise,
Rachel Parker,
Matthew J. J. Rose-Zerilli,
David M. Layfield,
Oliver Wood,
Jonathan West,
Christian H. Ottensmeier,
Gareth J. Thomas and
Christopher J. Hanley,
date: 12/05/2019,
view: 617,
Q&A: 0
[Abstract] Despite their involvement in many physiological and pathological processes, fibroblasts remain a poorly-characterized cell type. Analysis of primary fibroblasts while maintaining their in vivo phenotype is challenging: standard methods for fibroblast isolation require cell culture in vitro, which is known to alter phenotypes. ...
DNase I Chromatin Accessibility Analysis
Authors: Brook S. Nepon-Sixt and
Mark G. Alexandrow,
date: 12/05/2019,
view: 331,
Q&A: 0
[Abstract] Chromatin consists of compacted DNA in complex with proteins and contributes to the organization of DNA and its stability. Furthermore, chromatin plays key roles in regulating cellular processes such as DNA replication, transcription, DNA repair, and mitosis. Chromatin assumes more compact (inaccessible) or decondensed (accessible) conformations ...