Detection of Cell Death in Planarians
Authors: Nídia de Sousa and
Teresa Adell,
date: 10/05/2018,
view: 253,
Q&A: 0
[Abstract] Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the ...
Qualitative in vivo Bioluminescence Imaging
[Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the ...
Zebrafish Embryo Xenograft and Metastasis Assay
[Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
Cell Synchronization by Double Thymidine Block
Authors: Guo Chen and
Xingming Deng,
date: 09/05/2018,
view: 948,
Q&A: 0
[Abstract] Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at ...
Microarray, IPA and GSEA Analysis in Mice Models
[Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...
Hypoxia Reporter Element Assay
Authors: Daelynn R Buelow and
Sharyn D. D Baker,
date: 08/05/2018,
view: 381,
Q&A: 0
[Abstract] Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the ...
Mouse Mammary Gland Whole Mount Preparation and Analysis
Authors: Cornelia Tolg,
Mary Cowman and
Eva A. Turley,
date: 07/05/2018,
view: 1136,
Q&A: 0
[Abstract] The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure a method is needed that provides an overview of the entire gland. Whole mounts provide this ...