Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase
[Abstract] Almost all functions of cells or organs rely on the activities of cellular enzymes. Indeed, the in-vivo activities that directly represent the cellular effects of enzymes in live organs are critical importance to appreciate the roles enzymes play in modulating physiological or pathological processes, although assessments of such in-vivo ...
Visualization of Macropinocytosis in Prostate Fibroblasts
Authors: Rajeev Mishra and
Neil A. Bhowmick,
date: 05/20/2019,
view: 1320,
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[Abstract] Macropinocytosis has emerged as an important mechanism for non-selective route to internalize extracellular fluids and dissolved molecules in eukaryotic cell. As fundamental cellular behavior, macropinocytosis plays specific and distinct roles in many physiological and pathological processes, such as nutrients uptake, antigen presentation, ...
Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice
[Abstract] Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...
In situ, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses
Authors: Katrin Hufnagel,
Dennis Reininger,
Siu Wang Ng,
Nadine Gassert,
Juliane K. Rohland,
Soroosh Shahryarhesami,
Andrea S. Bauer,
Tim Waterboer and
Jörg D. Hoheisel,
date: 02/05/2019,
view: 911,
Q&A: 0
[Abstract] Until recently, whole-proteome microarrays for comprehensive studies of protein interactions were mostly produced by individual cloning and cellular expression of very many open reading frames, followed by protein isolation and purification as well as array production. To overcome this cumbersome process, we have developed a method to generate ...