Adoptive Transfer of Monocytes Sorted from Bone Marrow
[Abstract] Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to ...
Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
Authors: Selene K. Roberts,
Michael Hirsch,
Alexandra McStea,
Laura C. Zanetti-Domingues,
David T. Clarke,
Jeroen Claus,
Peter J. Parker,
Lin Wang and
Marisa L. Martin-Fernande,
date: 12/05/2018,
view: 292,
Q&A: 0
[Abstract] The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is overexpressed in many tumors, and the deregulated proliferation of cancerous cells is driven by cooperation with ...
In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
[Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.
Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...
Detection of Cell Death in Planarians
Authors: Nídia de Sousa and
Teresa Adell,
date: 10/05/2018,
view: 626,
Q&A: 0
[Abstract] Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the ...
Qualitative in vivo Bioluminescence Imaging
[Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the ...