Microbiology

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    Protocols in Current Issue
    Proteomics Profiling of S-sulfurated Proteins in Acinetobacter baumannii
    Authors:  Brenna J. C. Walsh and David P. Giedroc, date: 05/05/2021, view: 243, Q&A: 0

    Hydrogen sulfide (H2S) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species

    ...
    High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria
    Authors:  ShaoHua Gu, Wen Wan, ZhengYing Shao and Wei Zhong, date: 05/05/2021, view: 220, Q&A: 0

    Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated

    ...
    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    Author:  Mansoor A. Siddiqui, date: 05/05/2021, view: 385, Q&A: 0

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases

    ...
    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and

    ...
    Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting
    Authors:  Annemarie Voorberg-van der Wel, Sam O. Hoffman and Clemens H. M. Kocken, date: 05/05/2021, view: 194, Q&A: 0

    Hypnozoites are dormant liver-stage parasites unique to relapsing malarial species, including the important human pathogen Plasmodium vivax, and pose a barrier to the elimination of malaria. Little is known regarding the biology of these stages,

    ...
    Assessing Swarming of Aerobic Bacteria from Human Fecal Matter
    Authors:  Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani, date: 05/05/2021, view: 200, Q&A: 0

    Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood;

    ...
    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    Authors:  Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson, date: 05/05/2021, view: 181, Q&A: 0

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by

    ...
    Inkjet 3D Printing of Polymers Resistant to Fungal Attachment
    Authors:  Yinfeng He, Cindy Vallieres, Morgan R. Alexander, Ricky D. Wildman and Simon V. Avery, date: 05/05/2021, view: 313, Q&A: 0

    Inkjet 3D printing is an additive manufacturing method that allows the user to produce a small batch of customized devices for comparative study versus commercial products. Here, we describe the use of a commercial 2D ink development system

    ...
    Proteomics Profiling of S-sulfurated Proteins in Acinetobacter baumannii
    Authors:  Brenna J. C. Walsh and David P. Giedroc, date: 05/05/2021, view: 243, Q&A: 0
    [Abstract]

    Hydrogen sulfide (H2S) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species (RSS), derived from H2S. One mechanism by which H2S/RSS may signal in the cell is through proteome

    ...
    High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria
    Authors:  ShaoHua Gu, Wen Wan, ZhengYing Shao and Wei Zhong, date: 05/05/2021, view: 220, Q&A: 0
    [Abstract]

    Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by

    ...
    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    Author:  Mansoor A. Siddiqui, date: 05/05/2021, view: 385, Q&A: 0
    [Abstract]

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

    ...
    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

    ...
    Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting
    Authors:  Annemarie Voorberg-van der Wel, Sam O. Hoffman and Clemens H. M. Kocken, date: 05/05/2021, view: 194, Q&A: 0
    [Abstract]

    Hypnozoites are dormant liver-stage parasites unique to relapsing malarial species, including the important human pathogen Plasmodium vivax, and pose a barrier to the elimination of malaria. Little is known regarding the biology of these stages, largely due to their inaccessible location. Hypnozoites can be cultured in vitro but these cultures

    ...
    Assessing Swarming of Aerobic Bacteria from Human Fecal Matter
    Authors:  Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani, date: 05/05/2021, view: 200, Q&A: 0
    [Abstract]

    Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize

    ...
    Histological Methods to Detect Early-stage Plant Defense Responses during Artificial Inoculation of Lolium perenne with Epichloë festucae
    Authors:  Mostafa Rahnama, Damien J. Fleetwood and Richard D. Johnson, date: 05/05/2021, view: 181, Q&A: 0
    [Abstract]

    Epichloë species form agriculturally important symbioses with many cool season grasses. To study these symbioses, such as the interaction of Epichloë festucae with perennial ryegrass (Lolium perenne), host plants can be infected by artificial inoculation of etiolated seedlings. This inoculation is performed by placing mycelium into an

    ...
    Inkjet 3D Printing of Polymers Resistant to Fungal Attachment
    Authors:  Yinfeng He, Cindy Vallieres, Morgan R. Alexander, Ricky D. Wildman and Simon V. Avery, date: 05/05/2021, view: 313, Q&A: 0
    [Abstract]

    Inkjet 3D printing is an additive manufacturing method that allows the user to produce a small batch of customized devices for comparative study versus commercial products. Here, we describe the use of a commercial 2D ink development system (Dimatix material printing) to manufacture small batches of 3D medical or other devices using a recently

    ...
    A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
    Authors:  Benno Zehnder, Stephan Urban and Thomas Tu, date: 04/20/2021, view: 583, Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral transcripts. Specific and accurate quantification of cccDNA is difficult because infected cells contain

    ...
    Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
    Authors:  Javier Aguilera and Jianjun Sun, date: 04/20/2021, view: 499, Q&A: 0
    [Abstract] The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, the substrate of β-lactamase. The exogenously added, neutral charged-FRET reagent can diffuse across ...



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