Arthropods

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    Protocols in Current Issue
    Optogenetic Inactivation of Transcription Factors in the Early Embryo of Drosophila
    Authors:  Stephen L. McDaniel and Melissa M. Harrison, date: 07/05/2019, view: 602, Q&A: 0
    [Abstract] The early embryo of Drosophila melanogaster exists as a rapidly dividing syncytium of nuclei that are transcriptionally silent. Maternally deposited factors are required to awaken the genome and assist in the transition from maternal to zygotic control of development. Because many of these essential factors are maternally deposited and ...
    CRISPR-Cas9 Mediated Genome Editing in Drosophila
    Authors:  Ping Peng, Xia Wang, Da Shen, Jin Sun, Yu Jia, Rong-Gang Xu, Li-Fei Zhu and Jian-Quan Ni , date: 01/20/2019, view: 1945, Q&A: 0
    [Abstract] In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we describe a CRISPR-Cas9 mediated mutation protocol for efficiently and specifically editing genes in Drosophila. In this optimized system, the ...
    Chromatin Immunoprecipitation Experiments from Whole Drosophila Embryos or Larval Imaginal Discs
    [Abstract] Chromatin Immunoprecipitation coupled either to qPCR (qChIP) or high-throughput sequencing (ChIP-Seq) has been extensively used in the last decades to identify the DNA binding sites of transcription factors or the localization of various histone marks along the genome. The ChIP experiment generally includes 7 steps: collection of biological ...
    Protein Extraction from Drosophila Embryos and Ovaries
    Authors:  Pedro Prudêncio and Leonardo G. Guilgur, date: 05/05/2015, view: 11833, Q&A: 0
    [Abstract] Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos or ovaries are homogenized using a pestle and then the soluble proteins separated by ...



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