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    Protocols in Current Issue
    RNA-dependent RNA Polymerase Assay for Hepatitis E Virus
    Authors:  Vidya P. Nair, Saumya Anang, Akriti Srivastava and Milan Surjit, date: 04/05/2017, view: 5017, Q&A: 0
    [Abstract] RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome. This assay measures activity of the hepatitis E virus (HEV) RdRp. In contrast to protocols available to ...
    RNA Strand Displacement Assay for Hepatitis E Virus Helicase
    Authors:  Vidya P. Nair and Milan Surjit, date: 04/05/2017, view: 4635, Q&A: 0
    [Abstract] The hepatitis E virus (HEV) helicase uses ATP to unwind the RNA duplexes. This is an essential step for viral replication. This protocol aims to measure the double strand RNA unwinding activity of the HEV helicase.
    Luminescence-based Antiviral Assay for Hepatitis E Virus
    Authors:  Yannick Debing, Kai Dallmeier and Johan Neyts, date: 08/05/2014, view: 6467, Q&A: 0
    [Abstract] Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate ...
    Infectious Virus Yield Assay for Hepatitis E Virus
    Authors:  Yannick Debing, Kai Dallmeier and Johan Neyts, date: 08/05/2014, view: 6868, Q&A: 0
    [Abstract] Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate ...



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