Transient Expression Assay in Strawberry Fruits
Authors: Mengting Pi,
Qi Gao and
Chunying Kang,
date: 06/05/2019,
view: 182,
Q&A: 0
[Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...
Tracking Root Interactions System (TRIS) Experiment and Quality Control
[Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
Quantification of the Humidity Effect on HR by Ion Leakage Assay
Authors: Musoki Mwimba and
Xinnian Dong,
date: 04/05/2019,
view: 488,
Q&A: 0
[Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
Extraction and Purification of Laccases from Rice Stems
Authors: Chenna Swetha and
P. V. Shivaprasad,
date: 04/05/2019,
view: 468,
Q&A: 0
[Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...