Extraction and Purification of Laccases from Rice Stems
Authors: Chenna Swetha and
P. V. Shivaprasad,
date: 04/05/2019,
view: 3081,
Q&A: 0
[Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...
Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE
[Abstract] The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system. Mild non-ionic detergent digitonin is used to solubilize labile protein super-and megacomplexes, which are then separated with first-dimension blue native polyacrylamide gel electrophoresis ...
Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen
[Abstract] We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched ...
Rapid Isolation of Total Protein from Arabidopsis Pollen
Authors: Ming Chang and
Shanjin Huang,
date: 04/20/2017,
view: 7328,
Q&A: 0
[Abstract] Arabidopsis pollen is an excellent system for answering important biological questions about the establishment and maintenance of cellular polarity and polar cell growth, because these processes are amenable to the genetic and genomic approaches that are readily available in Arabidopsis. Given that proteins are the direct ...