Cancer Biology

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    Protocols in Current Issue
    Fat Turnover Assay in Drosophila
    Authors:  Subhash D. Katewa and Pankaj Kapahi, date: 11/05/2016, view: 8100, Q&A: 0
    [Abstract] Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of triglycerides in Drosophila.
    Quantification of 2-Hydroxyglutarate Enantiomers by Liquid Chromatography-mass Spectrometry
    Authors:  William M. Oldham and Joseph Loscalzo, date: 08/20/2016, view: 7699, Q&A: 0
    [Abstract] Two enantiomers of 2-hydroxyglutarate (2HG), L (L2HG) and D (D2HG), are metabolites of unknown function in mammalian cells that were initially associated with separate and rare inborn errors of metabolism resulting in increased urinary excretion of 2HG linked to neurological deficits in children (Chalmers et al., 1980; Duran et al ...
    Measurement of 2-methylthio Modifications in Mitochondrial Transfer RNAs by Reverse-transcription Quantitative PCR
    Authors:  Fan-Yan Wei and Kazuhito Tomizawa, date: 01/05/2016, view: 5874, Q&A: 0
    [Abstract] 2-Methylthio-N6-isopentenyladenosine (ms2i6A) is an evolutionally conserved posttranscriptional modification found at position 37 of four mammalian mitochondrial tRNAs, mt-tRNASer(UCN), mt-tRNATrp, mt-tRNAPhe and mt-tRNATyr. The ms2 modification in ms2 ...



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