Cancer Biology


    Protocols in Current Issue
    Generation of Tumour-stroma Minispheroids for Drug Efficacy Testing
    Authors:  Mark Watters and Eva Szegezdi, date: 01/05/2017, view: 6179, Q&A: 0
    [Abstract] The three-dimensional organisation of cells in a tissue and their interaction with adjacent cells and extracellular matrix is a key determinant of cellular responses, including how tumour cells respond to stress conditions or therapeutic drugs (Elliott and Yuan, 2011). In vivo, tumour cells are embedded in a stroma formed primarily by ...
    Preparation of Recombinant Galectin-3 for Cancer Studies
    Authors:  Kari Tyler, Sok-Hyong Lee and Erwin G. Van Meir, date: 01/05/2016, view: 6754, Q&A: 0
    [Abstract] Galectin-3 is a member of a class of proteins termed Galectins, characterized by their ability to bind glycans containing β-galactose (Cummings and Liu, 2009). Galectin-3 binds preferentially to proteoglycans terminating with N-acetyllactosamine (LacNAc) chains (i.e., tandem repeats of galactose) (Newlaczyl and Yu, 2011). Galectin-3 is ...
    Three Dimensional Spheroid Co-culture Invasion Assay
    Authors:  Eldo T Verghese and Thomas A Hughes, date: 01/05/2015, view: 10248, Q&A: 0
    [Abstract] The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional ...

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