Cancer Biology

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    Protocols in Current Issue
    Nucleosome Positioning Assay
    Authors:  Zhongliang Zhao and Holger Bierhoff, date: 05/20/2017, view: 8021, Q&A: 0
    [Abstract] The basic unit of chromatin is the nucleosome, a histone octamer with 147 base pairs of DNA wrapped around it. Positions of nucleosomes relative to each other and to DNA elements have a strong impact on chromatin structure and gene activity and are tightly regulated at multiple levels, i.e., DNA sequence, transcription factor binding, ...
    Neutral Comet Assay
    Authors:  Elisa Boutet-Robinet, Didier Trouche and Yvan Canitrot, date: 09/20/2013, view: 28298, Q&A: 1
    [Abstract] The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding ...
    End-synapsis Assay
    Authors:  Jessica Cottarel and Patrick Calsou, date: 09/20/2013, view: 6775, Q&A: 0
    [Abstract] Many environmental agents induce double-strand breaks (DSBs) in DNA. Unrepaired or improperly repaired DSBs can lead to cell death or cancer. Nonhomologous end joining is the primary DNA double-strand break repair pathway in eukaryotes. During NHEJ pathway, several proteins recognize and bind DNA ends, bring the ends in a synaptic complex and, ...
    Genomic 8-oxo-7,8-dihydro-2'-deoxyguanosine Quantification
    Author:  Antonio Sarno, date: 09/05/2013, view: 7001, Q&A: 0
    [Abstract] 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) is among the most common reactive oxygen species-induced DNA lesions and can be used as a biomarker for oxidative stress. The lesion has been linked to several biological processes and diseases, including colorectal cancer, Huntington’s disease, estrogen-induced gene expression, and thymine dimer ...
    Quantitative Methylation Specific PCR (qMSP)
    Authors:  Triantafillos Liloglou and Georgios Nikolaidis, date: 08/20/2013, view: 25326, Q&A: 0
    [Abstract] Detection of low copies of methylated DNA targets in clinical specimens is challenging. The quantitative Methylation-Specific PCR (qMSP) assays were designed to specifically amplify bisulphite-converted methylated DNA target sequences in the presence of an excess of unmethylated counterpart sequences. These qMSP assays are real-time PCR assays ...



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