Cancer Biology


    Protocols in Current Issue
    Nucleosome Positioning Assay
    Authors:  Zhongliang Zhao and Holger Bierhoff, date: 05/20/2017, view: 9323, Q&A: 0
    [Abstract] The basic unit of chromatin is the nucleosome, a histone octamer with 147 base pairs of DNA wrapped around it. Positions of nucleosomes relative to each other and to DNA elements have a strong impact on chromatin structure and gene activity and are tightly regulated at multiple levels, i.e., DNA sequence, transcription factor binding, ...
    Neutral Comet Assay
    Authors:  Elisa Boutet-Robinet, Didier Trouche and Yvan Canitrot, date: 09/20/2013, view: 31959, Q&A: 1
    [Abstract] The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding ...
    End-synapsis Assay
    Authors:  Jessica Cottarel and Patrick Calsou, date: 09/20/2013, view: 7350, Q&A: 0
    [Abstract] Many environmental agents induce double-strand breaks (DSBs) in DNA. Unrepaired or improperly repaired DSBs can lead to cell death or cancer. Nonhomologous end joining is the primary DNA double-strand break repair pathway in eukaryotes. During NHEJ pathway, several proteins recognize and bind DNA ends, bring the ends in a synaptic complex and, ...
    Genomic 8-oxo-7,8-dihydro-2'-deoxyguanosine Quantification
    Author:  Antonio Sarno, date: 09/05/2013, view: 7700, Q&A: 0
    [Abstract] 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) is among the most common reactive oxygen species-induced DNA lesions and can be used as a biomarker for oxidative stress. The lesion has been linked to several biological processes and diseases, including colorectal cancer, Huntington’s disease, estrogen-induced gene expression, and thymine dimer ...
    Quantitative Methylation Specific PCR (qMSP)
    Authors:  Triantafillos Liloglou and Georgios Nikolaidis, date: 08/20/2013, view: 28282, Q&A: 0
    [Abstract] Detection of low copies of methylated DNA targets in clinical specimens is challenging. The quantitative Methylation-Specific PCR (qMSP) assays were designed to specifically amplify bisulphite-converted methylated DNA target sequences in the presence of an excess of unmethylated counterpart sequences. These qMSP assays are real-time PCR assays ...

    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.