Cell Biology

Categories

    Protocols in Current Issue
    Analysis of B Cell Migration by Intravital Microscopy
    [Abstract]

    During immune responses, B cells home to lymph nodes (LN), where they encounter antigens. Homing starts with capture and L-selectin-dependent rolling on the activated endothelium of high endothelial venules (HEV). After recognition of chemokines presented on HEV, activation of B cell integrins occurs mediating firm arrest. Subsequently, B cells

    ...
    Preparation of Actinoplanes missouriensis Zoospores and Assay for Their Adherence to Solid Surfaces
    Authors:  Takeaki Tezuka, Daisuke Nakane, Tomohiro Kimura and Yasuo Ohnishi, date: 12/20/2019, view: 2023, Q&A: 0
    [Abstract] Spherical zoospores of a rare actinomycete, Actinoplanes missouriensis, adhere to various hydrophobic solid surfaces by means of type IV pili. The purpose of this protocol is to provide detailed descriptions of the preparation of A. missouriensis zoospores and an assay for the adhesion of the zoospores to solid surfaces. This ...
    Adhesion Assay for Murine Bone Marrow Hematopoietic Stem Cells
    Authors:  Seymen Avci, Shiri Gur-Cohen, Francesca Avemaria and Tsvee Lapidot, date: 02/20/2017, view: 8478, Q&A: 0
    [Abstract] Hematopoietic stem cells (HSCs) are defined by their functional abilities to self-renew and to give rise to all mature blood and immune cell types throughout life. Most HSCs are retained in a non-motile quiescent state within a specialized protective microenvironment in the bone marrow (BM) termed the niche. HSCs are typically distinguished from ...
    Bone Marrow-derived Endothelial Progenitor Cell Intercellular Adhesion Assay
    Authors:  Yubo Tang, Angela Jacobi, Corina Vater, Xuenong Zou and Maik Stiehler, date: 08/20/2016, view: 8285, Q&A: 0
    [Abstract] Cell-cell adhesion ensures tight contacts between neighbouring cells, which is necessary for cell segregation, as well as for the morphological and functional differentiation of different tissues. Evidently there are cell-cell recognition systems that make cells of the same type preferentially adherent to one another. Homotypic cell adhesion is ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.