Microbiology

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    Protocols in Current Issue
    Determination of Intracellular Osmolytes in Cyanobacterial Cells
    Authors:  Xiaoming Tan, Kuo Song, Cuncun Qiao and Xuefeng Lu, date: 04/20/2018, view: 3252, Q&A: 0
    [Abstract] Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of ...
    Determination of Survival of Wildtype and Mutant Escherichia coli in Soil
    Authors:  Yinka Somorin and Conor O'Byrne, date: 07/20/2017, view: 5078, Q&A: 0
    [Abstract] E. coli resides in the gastrointestinal tract of humans and other warm-blooded animals but recent studies have shown that E. coli can persist and grow in various external environments including soil. The general stress response regulator, RpoS, helps E. coli overcome various stresses, however its role in soil survival ...
    Enriching Acidophilic Fe(II)-oxidizing Bacteria in No-flow, Fed-batch Systems
    Authors:  Yizhi Sheng, Bradley Kaley and William D. Burgos, date: 02/05/2017, view: 6705, Q&A: 0
    [Abstract] Low-pH microbial Fe(II) oxidation occurs naturally in some Fe(II)-rich acid mine drainage (AMD) ecosystems across so-called terraced iron formations. Indigenous acidophilic Fe(II)-oxidizing bacterial communities can be incorporated into both passive and active treatments to remove Fe from the AMD solution. Here, we present a protocol of enriching ...
    Calculation of Microorganism Lag Times as a Measure of Adaptative Capability between Different Growth Conditions
    Author:  Brice Enjalbert, date: 02/05/2016, view: 4693, Q&A: 0
    [Abstract] This protocol has been designed as a simple and efficient way to investigate microorganism adaptive capabilities (Enjalbert et al., 2015). It is performed using switch experiments in which cells are initially grown in the first condition (primary cultures), then rapidly switched to the second condition (secondary culture) without ...



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