Cancer Biology

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    Protocols in Current Issue
    3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells
    Authors:  Lingli Long, Mingzhu Yin and Wang Min, date: 04/20/2018, view: 5415, Q&A: 0
    [Abstract] Ovarian cancer is fairly unique in that ovarian carcinoma cells can detach and spread directly through peritoneal cavity. It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure. We have recently reported that there is a strong correlation between Tumor-associated macrophages (TAMs)-associated ...
    Peptide Loading on MHC Class I Molecules of Tumor Cells
    Authors:  Loredana Cifaldi, Franco Locatelli and Doriana Fruci, date: 09/20/2016, view: 7233, Q&A: 0
    [Abstract] MHC class I molecules present peptides to cytotoxic T cells allowing the immune system to scan for intracellular pathogens and mutated proteins. The generation of antigenic peptides is a multistep process that ends in the endoplasmic reticulum (ER). Only peptides with the right length and sequence will bind nascent MHC class I molecules in the ER. ...
    Killer Cell Ig-like Receptors (KIR)-Binding Assay for Tumor Cells
    Authors:  Loredana Cifaldi, Franco Locatelli and Doriana Fruci, date: 09/20/2016, view: 4572, Q&A: 0
    [Abstract] Natural killer (NK) cells play key roles in innate and adaptive immune responses against virus and tumor cells. Their function relies on the dynamic balance between activating and inhibiting signals through receptors that bind ligands expressed on target cells. The absence of inhibitory receptor engagement with their ligands and the presence of ...
    Differentiation of THP1 Cells into Macrophages for Transwell Co-culture Assay with Melanoma Cells
    Authors:  Michael Peter Smith, Helen Young, Adam Hurlstone and Claudia Wellbrock, date: 11/05/2015, view: 32308, Q&A: 2
    [Abstract] Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combing both targeted- and immuno-therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be differentiated into macrophages. This allows studying ...
    In vitro Regulatory T cells Differentiation From Naïve T Cells
    Authors:  Tomás Dalotto-Moreno, Gabriel A. Rabinovich and Marian Salatino, date: 03/20/2014, view: 17680, Q&A: 1
    [Abstract] In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells is specifically engaged in the maintenance of immune self-tolerance and the control of aberrant ...



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