Cancer Biology

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    Protocols in Current Issue
    Calvarial Bone Implantation and in vivo Imaging of Tumor Cells in Mice
    Authors:  Kyoko Hashimoto, Shingo Sato, Hiroki Ochi, Shu Takeda and Mitsuru Futakuchi, date: 02/05/2019, view: 926, Q&A: 0
    [Abstract] Bone is one of common metastasis sites for many types of cancer. In bone metastatic microenvironment, tumor-bone interactions play a significant role in the regulation of osteolytic or osteoblastic bone metastasis. In order to investigate the direct interaction between tumor cells and bone tissue, it is essential to generate appropriate animal ...
    Qualitative in vivo Bioluminescence Imaging
    Authors:  Devbarna Sinha, Zalitha Pieterse and Pritinder Kaur, date: 09/20/2018, view: 1791, Q&A: 1
    [Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the ...
    Zebrafish Embryo Xenograft and Metastasis Assay
    Authors:  Ilkka Paatero, Sanni Alve, Silvia Gramolelli, Johanna Ivaska and Päivi M. Ojala, date: 09/20/2018, view: 2237, Q&A: 1
    [Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
    Whole Mammary Gland Transplantation in Mice Protocol
    Authors:  Hayley Hansford, Yan Hong, Frances Kittrell, Daniel Medina and Fariba Behbod, date: 06/05/2017, view: 3959, Q&A: 0
    [Abstract] Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it ...
    Protocol for Murine/Mouse Platelets Isolation and Their Reintroduction in vivo
    Authors:  Jae Hong Im and Ruth J. Muschel, date: 02/20/2017, view: 8888, Q&A: 1
    [Abstract] Platelets and coagulation have long been known to be essential for metastasis in experimental models. In order to study the interactions between tumor cells, platelets and endothelium, we have adapted methods used in coagulation research for the isolation of platelets and their reintroduction into mice. Anti-coagulated murine blood served as the ...
    A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis
    Authors:  Robert M. Hughes, Brian W. Simons and Paula J. Hurley, date: 02/20/2017, view: 5400, Q&A: 0
    [Abstract] Prostate cancer is one of the most common cancers in men in the United States. Comprehensive understanding of the biology contributing to prostate cancer will have important clinical implications. Animal models have greatly impacted our knowledge of disease and will continue to be a valuable resource for future studies. Herein, we describe a ...
    Establishment of Patient-Derived Xenografts in Mice
    Authors:  Dongkyoo Park, Dongsheng Wang, Guo Chen and Xingming Deng, date: 11/20/2016, view: 7423, Q&A: 0
    [Abstract] Patient-derived xenograft (PDX) models for cancer research have recently attracted considerable attention in both the academy and industry (Hidalgo et al., 2014; Wilding and Bodmer, 2014). PDX models have been developed from different tumor types including lung cancer to improve the drug development process. These models are used for ...
    Mouse Mammary Intraductal (MIND) Method for Transplantation of Patient Derived Primary DCIS Cells and Cell Lines
    Authors:  Frances Kittrell, Kelli Valdez, Hanan Elsarraj, Yan Hong, Daniel Medina and Fariba Behbod, date: 03/05/2016, view: 5580, Q&A: 0
    [Abstract] The MIND method involves intraductal injection of patient derived ductal carcinoma in situ (DCIS) cells and DCIS cell lines (MCF10DCIS.COM and SUM225) inside the mouse mammary ducts [Video 1 and Figure 1 in Behbod et al. (2009)]. This method mimics the normal environment of DCIS and facilitates study of the natural progression of human ...
    Immunostaining Protocol: P-Smad2 (Xenograft and Mice)
    [Abstract] Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-beta responses when injected into nude mice as shown by lack of p-SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we ...
    In vivo Extravasation Assay
    Authors:  Elisa Penna and Daniela Taverna, date: 02/20/2014, view: 8628, Q&A: 1
    [Abstract] Tumor metastases develop when disseminated intravascular cancer cells acquire the ability to arrest by adhering to the capillary walls of distant organs, actively extravasate into their parenchyma, proliferate and establish secondary colonies. The extravasation assay described here is an in vivo technique aimed to analyze the ability of ...



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