Biochemistry

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    Protocols in Current Issue
    Pyridine Hemochromagen Assay for Determining the Concentration of Heme in Purified Protein Solutions
    Authors:  Ian Barr and Feng Guo, date: 09/20/2015, view: 25534, Q&A: 2
    [Abstract] Heme is a common cofactor in proteins, found in hemoglobin, myoglobin, cytochrome P450, DGCR8, and nitric oxide synthase, among others. This protocol describes a method for quantifying heme that works best in purified protein samples. This protocol might be used to, for example, determine whether a given heme-binding protein is fully occupied by ...



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