Molecular Biology


    Protocols in Current Issue
    Structural Alignment and Covariation Analysis of RNA Sequences
    Authors:  Nicolas J. Tourasse and Fabien Darfeuille, date: 02/05/2020, view: 1463, Q&A: 0
    [Abstract] RNA molecules adopt defined structural conformations that are essential to exert their function. During the course of evolution, the structure of a given RNA can be maintained via compensatory base-pair changes that occur among covarying nucleotides in paired regions. Therefore, for comparative, structural, and evolutionary studies of RNA ...
    Post-crystallization Improvement of RNA Crystals by Synergistic Ion Exchange and Dehydration
    Authors:  Jinwei Zhang and Adrian R. Ferré-D’Amaré, date: 09/05/2015, view: 5937, Q&A: 0
    [Abstract] Compared to the recent dramatic growth in the numbers of genome-wide and functional studies of complex non-coding RNAs, mechanistic and structural analyses have lagged behind. A major technical bottleneck in the structural determination of large RNAs and their complexes is preparation of diffracting crystals. Empirically, a vast majority of such ...
    Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
    [Abstract] This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical modification of flexible and therefore possibly single-stranded nucleotides in a sequence-independent ...

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