Primary Cultures from Human GH-secreting or Clinically Non-functioning Pituitary Adenomas
[Abstract] Pituitary adenomas are among the more frequent intracranial tumors usually treated with both surgical and pharmacological–based on somatostatin and dopamine agonists–approaches. Although mostly benign tumors, the occurrence of invasive behaviors is often detected resulting in poorer prognosis. The use of primary cultures from human pituitary ...
Bacterial Survival in Dictyostelium
Authors: Regin Rønn,
Xiuli Hao,
Freja Lüthje,
Nadezhda A. German,
Xuanji Li,
Fuyi Huang,
Javan Kisaka,
David Huffman,
Hend A. Alwathnani,
Yong-Guan Zhu and
Christopher Rensing,
date: 07/05/2017,
view: 4015,
Q&A: 0
[Abstract] We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested ...
TGFβ Stimulation Assay
Author: Emanuel Rognoni,
date: 12/05/2014,
view: 9154,
Q&A: 0
[Abstract] TGFβ is part of a growth factor superfamily which modulates cell growth, differentiation, adhesion, migration, ECM synthesis and apoptosis (Massague, 1998; Siegel and Massague, 2003). Free TGFβ binds to its high affinity TGFβ receptor, a receptor serine/threonine kinase, inducing phosphorylation of Smad2/3 which subsequently forms a complex with ...
In vitro Regulatory T cells Differentiation From Naïve T Cells
[Abstract] In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells is specifically engaged in the maintenance of immune self-tolerance and the control of aberrant ...
In vivo BrdU Incorporation Assay for Murine Hematopioetic Stem Cells
Authors: Ningfei An and
Yubin Kang,
date: 11/05/2013,
view: 10814,
Q&A: 0
[Abstract] Bromodeoxyuridine (BrdU) is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. As such, BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period during which BrdU is available. The following protocol describes an in vivo BrdU incorporation assay as a measure ...
Chemosensitivity Assay
Authors: Haixia Zhang and
Yan Zhang,
date: 09/05/2013,
view: 7756,
Q&A: 0
[Abstract] Chemoresistance is one of the special properties of cancer stem cells, which is the main cause of chemotherapy failure and plays an important role in the recurrence of various cancers including osteosarcoma. The most widely used assay for evaluating chemoresistance is the modified cell proliferation assay. An equal number of cells are seeded onto ...
Scratch Wound Healing Assay
Author: Yanling Chen,
date: 03/05/2012,
view: 95452,
Q&A: 6
[Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” ...