Cell Biology


    Protocols in Current Issue
    Primary Cultures from Human GH-secreting or Clinically Non-functioning Pituitary Adenomas
    Authors:  Roberto Würth, Alessandra Pattarozzi, Federica Barbieri and Tullio Florio, date: 04/05/2018, view: 2240, Q&A: 0
    [Abstract] Pituitary adenomas are among the more frequent intracranial tumors usually treated with both surgical and pharmacological–based on somatostatin and dopamine agonists–approaches. Although mostly benign tumors, the occurrence of invasive behaviors is often detected resulting in poorer prognosis. The use of primary cultures from human pituitary ...
    Bacterial Survival in Dictyostelium
    [Abstract] We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested ...
    Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth
    Authors:  Natalia V. Andreeva and Alexander V. Belyavsky, date: 07/05/2017, view: 4171, Q&A: 0
    [Abstract] Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation. However, the effects of such protein layers are usually rather modest, which might be explained by the loss immobilized proteins ...
    Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
    Authors:  Esteban A. Orellana and Andrea L. Kasinski, date: 11/05/2016, view: 29774, Q&A: 0
    [Abstract] The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai and Kirtikara, 2006). This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be ...
    TGFβ Stimulation Assay
    Author:  Emanuel Rognoni, date: 12/05/2014, view: 9154, Q&A: 0
    [Abstract] TGFβ is part of a growth factor superfamily which modulates cell growth, differentiation, adhesion, migration, ECM synthesis and apoptosis (Massague, 1998; Siegel and Massague, 2003). Free TGFβ binds to its high affinity TGFβ receptor, a receptor serine/threonine kinase, inducing phosphorylation of Smad2/3 which subsequently forms a complex with ...
    In vitro Regulatory T cells Differentiation From Naïve T Cells
    Authors:  Tomás Dalotto-Moreno, Gabriel A. Rabinovich and Marian Salatino, date: 03/20/2014, view: 15209, Q&A: 1
    [Abstract] In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells is specifically engaged in the maintenance of immune self-tolerance and the control of aberrant ...
    In vivo BrdU Incorporation Assay for Murine Hematopioetic Stem Cells
    Authors:  Ningfei An and Yubin Kang, date: 11/05/2013, view: 10814, Q&A: 0
    [Abstract] Bromodeoxyuridine (BrdU) is a thymidine analog that is incorporated into DNA during the S-phase of the cell cycle. As such, BrdU incorporation can be used to quantify the number of cells that are in S-phase in the time period during which BrdU is available. The following protocol describes an in vivo BrdU incorporation assay as a measure ...
    Chemosensitivity Assay
    Authors:  Haixia Zhang and Yan Zhang, date: 09/05/2013, view: 7756, Q&A: 0
    [Abstract] Chemoresistance is one of the special properties of cancer stem cells, which is the main cause of chemotherapy failure and plays an important role in the recurrence of various cancers including osteosarcoma. The most widely used assay for evaluating chemoresistance is the modified cell proliferation assay. An equal number of cells are seeded onto ...
    Scratch Wound Healing Assay
    Author:  Yanling Chen, date: 03/05/2012, view: 95452, Q&A: 6
    [Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” ...

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