Cell Biology

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    Protocols in Current Issue
    Determination of the Cellular Ion Concentration in Saccharomyces cerevisiae Using ICP-AES
    Authors:  Louise Thines, Anne Iserentant and Pierre Morsomme, date: 08/20/2020, view: 598, Q&A: 0
    [Abstract] The yeast Saccharomyces cerevisiae has been perceived over decades as a highly valuable model organism for the investigation of ion homeostasis. Indeed, many of the genes and biological systems that function in yeast ion homeostasis are conserved throughout unicellular eukaryotes to humans. In this context, measurement of the yeast ...
    MAMP-triggered Medium Alkalinization of Plant Cell Cultures
    [Abstract] Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the ...
    Analyzing the Quenchable Iron Pool in Murine Macrophages by Flow Cytometry
    Authors:  Michael Riedelberger and Karl Kuchler, date: 03/20/2020, view: 1143, Q&A: 0
    [Abstract] Tissue-resident macrophages are pivotal for a tightly-regulated iron metabolism at a cellular and systemic level, since subtle iron alterations increase the susceptibility for microbial infections or drive multiple diseases. However, research on cellular iron homeostasis in macrophages remains challenging due to the limited amount of available ...
    In vivo Live Imaging of Calcium Waves and Other Cellular Processes during Fertilization in Caenorhabditis elegans
    Authors:  Jun Takayama, Masashi Fujita and Shuichi Onami, date: 04/05/2017, view: 6527, Q&A: 0
    [Abstract] Fertilization calcium waves are a conserved trigger for animal development; however, genetic analysis of these waves has been limited due to the difficulty of imaging in vivo fertilization. Here we describe a protocol to image calcium dynamics during in vivo fertilization in the genetic animal model Caenorhabditis elegans ...
    Two-electrode Voltage-clamp Recordings in Xenopus laevis Oocytes:Reconstitution of Abscisic Acid Activation of SLAC1 Anion Channel via PYL9 ABA Receptor
    Authors:  Cun Wang, Jingbo Zhang and Julian I. Schroeder, date: 01/20/2017, view: 8062, Q&A: 0
    [Abstract] Two-Electrode Voltage-Clamp (TEVC) recording in Xenopus laevis oocytes provides a powerful method to investigate the functions and regulation of ion channel proteins. This approach provides a well-known tool to characterize ion channels or transporters expressed in Xenopus laevis oocytes. The plasma membrane of the oocyte is ...
    Measurement of H+ Flux in Rice by Non-invasive Micro-test Technology
    Authors:  Xi Zhang, Xiu-Yue Zhang and Li-Ping Yin, date: 12/05/2015, view: 6264, Q&A: 1
    [Abstract] Rice plants release proton (H+) from root cells into rhizosphere area leading to the acidification of the rhizosphere and increased solubility of ferric iron complexes on the cell membrane, which is important for iron uptakes. Here, we present a detailed protocol to measure H+ flux in root hairs of transgenic rice seedlings ...
    An Assay to Test the Capacity of Arabidopsis Plant Defensin Type1 Protein to Induce Cellular Zinc (Zn) Tolerance in Yeast
    Authors:  Zaigham Shahzad, Laurence Marquès, Pierre Berthomieu and Françoise Gosti, date: 11/20/2015, view: 5847, Q&A: 0
    [Abstract] Heterologous expression of genes in budding yeast Saccharomyces cerevisiae (S. cerevisiae) is especially suitable to functionally study the corresponding encoded protein at the cellular level (Bonneaud et al., 1991). This is mainly because many strains defective in specific activities are available and could be ...
    Loading of Cells with Fluorescent Probe to Study Intracellular Acid-base Homeostasis in Lactic Acid Bacteria
    Authors:  Pablo Mortera, Federico Zuljan, Christian Magni and Sergio H. Alarcón, date: 01/20/2015, view: 9384, Q&A: 0
    [Abstract] Here we describe a protocol which we have used to study the homeostasis intracellular in vivo in lactic acid bacteria (LAB) using a fluorescent probe. This type of probes can be used for determining changes in the pH of cytoplasm with high sensitivity, temporal resolution and technical simplicity as well as accessing the rate of change ...
    A Protocol for Measurement of Intracellular pH
    Authors:  Iman Saramipoor Behbahan, Matthew A. McBrian and Siavash K. Kurdistani, date: 01/20/2014, view: 17846, Q&A: 1
    [Abstract] Intracellular pH (pHi) is an important physiological determinant of enzyme activity and cellular function (Kurkdjian and Guern, 1989). All proteins depend on a tightly regulated pH to maintain their structure and function. Protonation–deprotonation events can dictate the charge of biological surfaces and are integral steps in many ...
    Transport Assays in Aspergillus nidulans
    Authors:  Emilia Krypotou and George Diallinas, date: 11/20/2013, view: 6376, Q&A: 0
    [Abstract] Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent Km and Vmax values, and permit the characterization of substrate specificity profiles through competition assays. In this protocol, we describe a rapid and easy method for performing uptake assays in the model ...



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