Stem Cell

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    Protocols in Current Issue
    Primary Cultures from Human GH-secreting or Clinically Non-functioning Pituitary Adenomas
    Authors:  Roberto Würth, Alessandra Pattarozzi, Federica Barbieri and Tullio Florio, date: 04/05/2018, view: 2778, Q&A: 0
    [Abstract] Pituitary adenomas are among the more frequent intracranial tumors usually treated with both surgical and pharmacological–based on somatostatin and dopamine agonists–approaches. Although mostly benign tumors, the occurrence of invasive behaviors is often detected resulting in poorer prognosis. The use of primary cultures from human pituitary ...
    Melanoma Stem Cell Sphere Formation Assay
    Authors:  Alessandra Tuccitto, Valeria Beretta, Francesca Rini, Chiara Castelli and Michela Perego, date: 04/20/2017, view: 7344, Q&A: 0
    [Abstract] Self-renewal is the ability of cells to replicate themselves at every cell cycle. Throughout self-renewal in normal tissue homeostasis, stem cell number is maintained constant throughout life. Cancer stem cells (CSCs) share this ability with normal tissue stem cells and the sphere formation assay (SFA) is the gold standard assay to assess stem ...
    Glioma Associated Stem Cells (GASCs) Isolation and Culture
    [Abstract] Glioma Associated Stem Cells (GASCs) represent a population of non-tumorigenic multipotent stem cells hosted in the microenvironment of human gliomas. In vitro, these cells are able, through the release of exosomes, to increase the biological aggressiveness of glioma-initiating cells. The clinical importance of this finding is supported ...
    In vivo Lineage-tracing Studies in a Cancer Stem Cell Population in Neuroblastoma
    Authors:  Danielle M. Hsu, Jason M. Shohet and Eugene S. Kim, date: 04/20/2014, view: 6836, Q&A: 0
    [Abstract] Tumors are comprised of heterogeneous subpopulations that may exhibit differing capacity for differentiation, self-renewal, and tumorigenicity. In vivo lineage-tracing studies are a powerful tool for defining the role of tumor subpopulations in tumor growth and as targets for therapeutic agents. This protocol describes using a ...



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