Home About Us

Cell Biology

High Quality Antibodies for Your Research

Latest Protocol Video

Non-separate Mouse Sclerochoroid/RPE/Retina Staining and Whole Mount for the Integral Observation of Subretinal Layer

Categories

Protocols in Current Issue

By Date By View

Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections

Authors: Kawssar Harb, Michele Bertacchi and Michèle Studer, date: 01/05/2021, view: 544, Q&A: 0

[Abstract]

The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded transcription factors and molecular determinants that define the identity of different projection

...

Use of Optogenetic Amyloid-β to Monitor Protein Aggregation in Drosophila melanogaster, Danio rerio and Caenorhabditis elegans

Authors: Prameet Kaur, Caroline Kibat, Emelyne Teo, Jan Gruber, Ajay Mathuru and Nicholas S. Tolwinski, date: 12/05/2020, view: 521, Q&A: 0

[Abstract]

Alzheimer’s Disease (AD) has long been associated with accumulation of extracellular amyloid plaques (Aβ) originating from the Amyloid Precursor Protein. Plaques have, however, been discovered in healthy individuals and not all AD brains show plaques, suggesting that extracellular Aβ aggregates may play a smaller role than

...

In vitro Time-lapse Imaging of Primary Cilium in Migrating Neuroblasts

Authors: Masato Sawada, Mami Matsumoto, Keishi Narita, Natsuko Kumamoto, Shinya Ugawa, Sen Takeda and Kazunobu Sawamoto, date: 11/20/2020, view: 615, Q&A: 0

[Abstract] Neuronal migration is a critical step for the development of neuronal circuits in the brain. Immature new neurons (neuroblasts) generated in the postnatal ventricular-subventricular zone (V-SVZ) show a remarkable potential to migrate for a long distance at a high speed in the postnatal mammalian brain, and are thus a powerful model to analyze the ...

Real-time Three-dimensional Tracking of Endocytic Vesicles

Authors: Stephanie Duhamel and Kossay Zaoui, date: 10/20/2020, view: 719, Q&A: 0

[Abstract] Endocytic trafficking and recycling are fundamental cellular processes that control essential functions such as signaling protein complexes transport and membrane identity. The small GTPase Rabs are indispensable component of the endosomal recycling machinery. The Rabs bind to effectors to mediate their functions, such as protein sorting and ...

Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli

Author: Sun-sang J. Sung, date: 07/05/2020, view: 1057, Q&A: 0

[Abstract] Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular dysfunction and destruction is an important disease mechanism. The cellular distribution and dynamics ...

Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidis–Arabidopsis

Authors: Fang-Yu Hwu and Martin Parniske, date: 06/20/2020, view: 1187, Q&A: 0

[Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...

Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration

Authors: Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans, date: 06/20/2020, view: 1285, Q&A: 0

[Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...

Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images

Authors: Akira Kitamura and Masakata Kinjo, date: 05/20/2020, view: 1350, Q&A: 0

[Abstract] Size determination of subcellular structures such as inclusion bodies (IBs) and granules from fluorescent images is important for identification and structural characterization. However, it is often time-consuming just for the comparison of the average size of the structures. Here, we introduce a high-throughput procedure to represent the average ...

Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System

Authors: Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger, date: 05/05/2020, view: 1813, Q&A: 0

[Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...

Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila

Authors: Océane Marescal, Frieder Schöck and Nicanor González-Morales, date: 04/05/2020, view: 1757, Q&A: 0

[Abstract] Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two ...

Find out more

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.