Real-time Three-dimensional Tracking of Endocytic Vesicles
Authors: Stephanie Duhamel and
Kossay Zaoui,
date: 10/20/2020,
view: 225,
Q&A: 0
[Abstract] Endocytic trafficking and recycling are fundamental cellular processes that control essential functions such as signaling protein complexes transport and membrane identity. The small GTPase Rabs are indispensable component of the endosomal recycling machinery. The Rabs bind to effectors to mediate their functions, such as protein sorting and ...
Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli
Author: Sun-sang J. Sung,
date: 07/05/2020,
view: 777,
Q&A: 0
[Abstract] Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular dysfunction and destruction is an important disease mechanism. The cellular distribution and dynamics ...
Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
[Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...
Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
[Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
Lipid Mixing Assay for Murine Myoblast Fusion and Other Slow Cell-cell Fusion Processes
[Abstract] Lipid mixing (redistribution of lipid probes between fusing membranes) has been widely used to study early stages of relatively fast viral and intracellular fusion processes that take seconds to minutes. Lipid mixing assays are especially important for identification of hemifusion intermediates operationally defined as lipid mixing without content ...
Quantification of Protein Enrichment at Plasmodesmata
[Abstract] Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell-to-cell communication takes place through nanometric membrane channels called plasmodesmata (PD). Understanding how PD dictate cellular connectivity greatly ...