Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
[Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
Increasing the Membrane Permeability of a Fern with DMSO
Author: Marcelo Garcés,
date: 06/20/2018,
view: 2628,
Q&A: 0
[Abstract] Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum fronds, we incubate fronds with 0.1% DMSO to test if this increases cell membrane permeability ...
Slow and Fast Desiccation of Single-cell Thick Fronds of Filmy Ferns
Author: Marcelo Garcés,
date: 06/05/2018,
view: 2606,
Q&A: 0
[Abstract] Filmy ferns can desiccate and recover after rehydration to resume photosynthesis. Slow and fast desiccation rates were compared in filmy fern fronds to determine whether structural or physiological differences may occur between desiccation rates. Slow desiccation is considered to be more similar to natural conditions experienced by plants that ...
Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images
[Abstract] The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of ...
Histochemical Preparations to Depict the Structure of Cauliflower Leaf Hydathodes
[Abstract] Hydathodes are plant organs present on leaf margins of a wide range of vascular plants and are the sites of guttation. Both anatomy and physiology of hydathodes are poorly documented. We have recently reported on the anatomy of cauliflower and Arabidopsis thaliana hydathodes and on their infection by the vascular pathogenic bacterium ...