Biochemistry

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    Protocols in Current Issue
    GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues
    Authors:  Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur, date: 09/20/2018, view: 2999, Q&A: 0
    [Abstract] Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising ...
    Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)
    Authors:  Anna Worthmann, Clara John and Joerg Heeren, date: 07/05/2018, view: 1643, Q&A: 0
    [Abstract] The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice ...
    Tracking Lipid Transfer by Fatty Acid Isotopolog Profiling from Host Plants to Arbuscular Mycorrhiza Fungi
    Authors:  Andreas Keymer, Claudia Huber, Wolfgang Eisenreich and Caroline Gutjahr, date: 04/05/2018, view: 2593, Q&A: 0
    [Abstract] Lipid transfer from host plants to arbuscular mycorrhiza fungi was hypothesized for several years because sequenced arbuscular mycorrhiza fungal genomes lack genes encoding cytosolic fatty acid synthase (Wewer et al., 2014; Rich et al., 2017). It was finally shown by two independent experimental approaches (Jiang et al., ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    Authors:  Motohide Aoki and Norihiro Sato, date: 01/05/2018, view: 3093, Q&A: 0
    [Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai ...
    Lipidomic Analysis of Caenorhabditis elegans Embryos
    Authors:  Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu, date: 09/20/2017, view: 4565, Q&A: 0
    [Abstract] Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is ...
    Sterol Analysis in Kluyveromyces lactis
    Authors:  Yvetta Gbelska, Nora Toth Hervay, Marcela Morvova and Alexandra Konecna, date: 09/05/2017, view: 3169, Q&A: 0
    [Abstract] Sterols are essential lipids of most eukaryotic cells with multiple functions (structural, regulatory and developmental). Sterol profile of yeast cells is often determined during the studies of ergosterol synthesis mutants used to uncover a number of functions for various sterols in yeast cells. Molecular studies of ergosterol biosynthesis have ...
    Metabolic Heavy Isotope Labeling to Study Glycerophospholipid Homeostasis of Cultured Cells
    Authors:  Satu Hänninen, Pentti Somerharju and Martin Hermansson, date: 05/05/2017, view: 3697, Q&A: 0
    [Abstract] Glycerophospholipids consist of a glycerophosphate backbone to which are esterified two acyl chains and a polar head group. The head group (e.g., choline, ethanolamine, serine or inositol) defines the glycerophospholipid class, while the acyl chains together with the head group define the glycerophospholipid molecular species. Stable ...
    Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
    Authors:  Tim Andrew Davies Smith and Su Myat Phyu, date: 11/20/2016, view: 4023, Q&A: 0
    [Abstract] The choline-containing phospholipid, phosphatidylcholine (PtdCho) is the most common mammalian phospholipid found in cell membrane (Ide et al., 2013). It is also a component of intracellular signalling pathways (Cui and Houweling, 2002). Herein is described a measure of the rate of accumulation of choline by lipid soluble PtdCho and ...
    Ionization Properties of Phospholipids Determined by Zeta Potential Measurements
    Authors:  Murugappan Sathappa and Nathan N. Alder, date: 11/20/2016, view: 7039, Q&A: 0
    [Abstract] Biological membranes are vital for diverse cellular functions such as maintaining cell and organelle structure, selective permeability, active transport, and signaling. The surface charge of the membrane bilayer plays a critical role in these myriad processes. For most biomembranes, the surface charge of anionic phospholipids contributes to the ...
    Fat Turnover Assay in Drosophila
    Authors:  Subhash D. Katewa and Pankaj Kapahi, date: 11/05/2016, view: 6495, Q&A: 0
    [Abstract] Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of triglycerides in Drosophila.



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