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Non-separate Mouse Sclerochoroid/RPE/Retina Staining and Whole Mount for the Integral Observation of Subretinal Layer

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Analysis of Monosaccharides from Arabidopsis Seed Mucilage and Whole Seeds Using HPAEC-PAD

Authors: Gillian H. Dean, Kresimir Sola, Faride Unda, Shawn D. Mansfield and George W. Haughn, date: 12/20/2019, view: 2107, Q&A: 0

[Abstract] Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development. When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed. Determining the monosaccharide composition of ...

Tensile Testing Assay for the Measurement of Tissue Stiffness in Arabidopsis Inflorescence Stem

Authors: Kouki Yoshida, Shingo Sakamoto and Nobutaka Mitsuda, date: 08/05/2019, view: 5668, Q&A: 1

[Abstract] Lignocellulosic biomass is a versatile renewable resource for fuels, buildings, crafts, and biomaterials. Strategies of molecularly designing lignocellulose for industrial application has been developed by the discoveries of novel genes after the screenings of various mutants and transformed lines of Arabidopsis whose cell walls could be ...

Fluorescein Transport Assay to Assess Bulk Flow of Molecules Through the Hypocotyl in Arabidopsis thaliana

Authors: Salva Duran-Nebreda and George W. Bassel, date: 04/05/2018, view: 4140, Q&A: 0

[Abstract] The bulk transport of molecules through plant tissues underpins growth and development. The stem acts as a conduit between the upper and low domains of the plant, facilitating transport of solutes and water from the roots to the shoot system, and sugar plus other elaborated metabolites towards the non-photosynthetic organs. In order to perform ...

Polyamine and Paraquat Transport Assays in Arabidopsis Seedling and Callus

Authors: Haoxi Chai, Yun Shen and Huazhong Shi, date: 08/05/2017, view: 4757, Q&A: 0

[Abstract] Polyamines (PAs) are polycationic compounds found in all living organisms and play crucial roles in growth and survival. We here show the ‘Polyamine and paraquat (PQ) transport assay’ protocol, which can be used to examine the uptake activity of PA/PQ transporters. We have used this protocol to demonstrate that PUT3 in Arabidopsis is a ...

Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints

Authors: Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 7998, Q&A: 2

[Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...

Measurement of Stomatal Conductance in Rice

Authors: Yin Wang and Toshinori Kinoshita, date: 04/20/2017, view: 9205, Q&A: 0

[Abstract] Stomatal conductance, the reciprocal of stomatal resistance, represents the gas exchange ability of stomata. Generally, the stomatal conductance is higher when stomata open wider, and vice versa. In this protocol, we describe how to measure stomatal conductance in rice using Li-6400 (Licor, USA).

Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants

Authors: Jian-Jun Tao, Shou-Yi Chen and Jin-Song Zhang, date: 09/05/2016, view: 8998, Q&A: 0

[Abstract] Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using ...

Measuring Auxin Transport Capacity in Seedling Roots of Medicago truncatula

Authors: Jason Liang Pin Ng and Ulrike Mathesius, date: 06/20/2016, view: 7074, Q&A: 0

[Abstract] Measurement of auxin transport capacity provides quantitative data on the physiological machinery involved in auxin transport within plants. This technique is easy to perform and gives quick results. Radiolabelled auxin (indole-3-acetic-acid) is fed into the roots of Medicago truncatula via an agar block. The resulting radioactivity from ...

Quantifying Auxin Metabolites in Young Root Tissue of Medicago truncatula by Liquid Chromatography Electrospray-ionisation Quadrupole Time-of-flight (LC-ESI-QTOF) Tandem Mass Spectrometry

Authors: Jason Liang Pin Ng, Thy T. Truong, Charles H. Hocart and Ulrike Mathesius, date: 06/20/2016, view: 6976, Q&A: 0

[Abstract] Auxins represent a major group of phytohormones controlling plant development. The spatio-temporal regulation of auxin gradients is essential for the initiation, growth and correct development of plant organs. Because auxins and their metabolites occur at trace levels in plant tissue, experiments requiring identification plus their selective and ...

Sample Preparation for X-ray Micro-computed Tomography of Woody Plant Material and Associated Xylem Visualisation Techniques

Author: Nicholas J. B. Brereton, date: 03/20/2016, view: 7138, Q&A: 0

[Abstract] Variation in the tissue structure of short rotation coppice (SRC) willow is a principle factor driving differences in lignocellulosic sugar yield yet much of the physiology and development of this tissue is unknown. Traditional sectioning can be both difficult and destructive in woody tissue; however, technology such as three dimensional X-ray ...

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