Stem Cell

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    Protocols in Current Issue
    Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
    Authors:  Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk, date: 02/20/2019, view: 1592, Q&A: 0
    [Abstract] For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell source for neural cell replacement but a useful model for developmental biology research. Despite ...
    Reprogram Murine Epiblast Stem Cells by Epigenetic Inhibitors
    Authors:  Hui Zhang and Yali Dou, date: 03/05/2017, view: 3795, Q&A: 0
    [Abstract] Pluripotent stem cells in the naïve state are highly useful in regenerative medicine and tissue engineering. A robust reprogramming of the primed murine Epiblast Stem Cells (EpiSCs) to naïve pluripotency is feasible via chemical-only approach. This protocol described a method to reprogram murine EpiSCs by MM-401 treatment, which blocks histone ...
    Differentiation of Human Embryonic Stem Cells into Cone Photoreceptors
    Authors:  Anthony Flamier, Andrea Barabino and Gilbert Bernier, date: 07/20/2016, view: 5585, Q&A: 1
    [Abstract] Photoreceptors are specialized retinal neurons able to respond to light in order to generate visual information. Among photoreceptors, cones are involved in colors discrimination and high-resolution central vision and are selectively depleted in macular degenerations and cone dystrophies. A possible therapeutic solution for these disorders is to ...
    Retinal Differentiation of Mouse Embryonic Stem Cells
    Author:  Anna La Torre, date: 07/05/2016, view: 8424, Q&A: 5
    [Abstract] Groundbreaking studies from Dr. Yoshiki Sasai’s laboratory have recently introduced novel methods to differentiate mouse and human Embryonic Stem Cells (mESCs and hESCs) into organ-like 3D structures aimed to recapitulate developmental organogenesis programs (Eiraku et al., 2011; Eiraku and Sasai, 2012; Nakano et al., 2012; ...
    Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation
    Authors:  Federica Pisano and Massimiliano Gnecchi, date: 02/05/2016, view: 5284, Q&A: 1
    [Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the efficiency of cardiac ...
    Wnt Reporter Activity Assay
    Author:  Chen Zhao, date: 07/20/2014, view: 22444, Q&A: 2
    [Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Wnt ligands or Wnt pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Wnt pathway responses. Canonical Wnt signaling activity transcriptionally induces Wnt ...
    Hedgehog (Hh) Reporter Activity Assay
    Author:  Chen Zhao, date: 07/20/2014, view: 10372, Q&A: 0
    [Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Hh ligands or Hh pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Hh pathway responses. Canonical Hh signaling activity transcriptionally induces Hh ...
    Intestinal Differentiation of Mouse ESCs
    Authors:  Soichiro Ogaki and Shoen Kume, date: 12/20/2013, view: 5351, Q&A: 0
    [Abstract] ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of ...
    Intestinal Differentiation of Human ESCs
    Authors:  Soichiro Ogaki and Shoen Kume, date: 12/20/2013, view: 5305, Q&A: 0
    [Abstract] ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of ...
    Mouse ESC Differentiation to Nkx2.1+ Lung and Thyroid Progenitors
    Authors:  Tyler A. Longmire, Laertis Ikonomou and Darrell N. Kotton, date: 11/20/2012, view: 8525, Q&A: 0
    [Abstract] The de novo derivation of lung progenitors from pluripotent stem cells provides the opportunity to model early lung development in vitro and allows easy access to cells for tissue engineering or basic cell biology studies. This detailed protocol allows the generation of lung and thyroid progenitors from mouse embryonic stem cell ...



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