Plant Science


    Protocols in Current Issue
    Atomic Force Microscopy to Characterize Ginger Lipid-Derived Nanoparticles (GLDNP)
    Authors:  Dingpei Long, Chunhua Yang, Junsik Sung and Didier Merlin, date: 04/05/2021, view: 160, Q&A: 0

    We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols).

    Chromatographic Analysis for Targeted Metabolomics of Antioxidant and Flavor-Related Metabolites in Tomato
    Authors:  Nergiz Gürbüz Çolak, Neslihan Tek Eken, Anne Frary and Sami Doğanlar, date: 03/05/2021, view: 783, Q&A: 0

    Targeted metabolomics is a useful approach to evaluate crop breeding studies. Antioxidant and flavor-related traits are of increasing interest and are considered quality traits in tomato breeding. The present study presents chromatographic methods to study antioxidants (carotenoids, vitamin C, vitamin E, phenolic compounds, and glutathione) and

    Using 14C-acetate Pulse-chase Labeling to Study Fatty Acid and Glycerolipid Metabolism in Plant Leaves
    Authors:  Linhui Yu, Chao Zhou, Jilian Fan and Changcheng Xu, date: 02/05/2021, view: 440, Q&A: 0

    Lipids metabolism is comprised of networks of reactions occurred in different subcellular compartments. Isotopic labeling is a good way to track the transformations and movements of metabolites without perturbing overall cellular metabolism. Fatty acids, the building blocks of membrane lipids and storage triacylglycerols, are synthesized in

    Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves
    Authors:  Hari Kiran Kotapati and Philip D. Bates, date: 12/20/2020, view: 652, Q&A: 0

    Plant lipid metabolism is a dynamic network where synthesis of essential membrane lipids overlaps with synthesis of valuable storage lipids (e.g., vegetable oils). Monogalactosyldiacylglycerol (MGDG) is a key component of the chloroplast membrane system required for photosynthesis and is produced by multiple pathways within the lipid metabolic

    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    Authors:  Keshun Yu, Huazhen Liu and Pradeep Kachroo, date: 12/05/2020, view: 884, Q&A: 0

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

    A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips
    Author:  Nguyen Hoai Nguyen, date: 10/05/2020, view: 1116, Q&A: 0
    [Abstract] Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...
    Determination of Ureides Content in Plant Tissues
    Author:  Ignacio Lescano, date: 06/05/2020, view: 1521, Q&A: 0
    [Abstract] The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N2 fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in ...
    Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
    Authors:  Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida, date: 01/05/2020, view: 2176, Q&A: 0
    [Abstract] Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca2+-dependent and redox-dependent signals. Conventional in ...
    Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues
    [Abstract] The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes ...

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