The Discrete Paired-trial Variable-delay T-maze Task to Assess Working Memory in Mice
[Abstract] Working memory abnormalities involving the prefrontal cortex (PFC) dramatically contribute to poor functional outcomes in patients with schizophrenia and still represent an unmet therapeutic need. Studies in rodents might provide essential tools to understand the mechanisms underlying PFC-dependent working memory dysfunctions, as well as precious ...
An Alternative Maze to Assess Novel Object Recognition in Mice
[Abstract] The novel object recognition (NOR) task is a behavioral test commonly used to evaluate episodic-like declarative memory and it relies on the innate tendency of rodents to explore novelty. Here we present a maze used to evaluate NOR memory in mice that reduces the time of the assay while improving reliability of the measurements by increasing the ...
Examining Cocaine Conditioning Place Preference in Mice
[Abstract] A key component of combating substance use disorders is understanding the neural mechanisms that support drug reward. Tasks such as self-administration assess the reinforcing properties of a drug using a learned behavior but require numerous training sessions and surgery. In comparison, the conditioned place preference (CPP) task assesses reward ...
A Simple and Fast Battery Test for Phenotypic Characterization of Mice
Authors: Mariana S. A. Garcia-Gomes,
Dennis A. Zanatto,
Pedro K. Yamamoto,
Danilo Wadt,
Ana T. F. B. Antiorio,
Jilma Aleman-Laporte,
Sandra R. Alexandre-Ribeiro,
Guilherme A. Marson,
Cezar Guizzo,
Silvia M. G. Massironi,
Maria M. Bernardi and
Claudia M. C. Mori,
date: 04/05/2020,
view: 559,
Q&A: 0
[Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
[Abstract] The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular environment. This biofluid contains an enriched source of proteins, ions, and other signaling molecules for ...
Implanting and Recycling Neuropixels Probes for Recordings in Freely Moving Mice
[Abstract] Recording neural activity in unrestricted animals is necessary to unravel the neural basis of ethological behaviors. Recently, Neuropixels probes have made important strides in improving yield and lowering noise, but have limited use cases in freely moving animals. Although there are a number of studies demonstrating the use of these probes in ...