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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

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Calcium Imaging of Neuronal Activity under Gradually Changing Odor Stimulation in Caenorhabditis elegans

Authors: Yuki Tanimoto and Koutarou D. Kimura, date: 01/05/2021, view: 581, Q&A: 0

[Abstract]

Olfactory behavior is among the most fundamental animal behaviors both in the wild and in the laboratory. To elucidate the neural mechanisms underlying olfactory behavior, it is critical to measure neural responses to odorant concentration changes resembling those that animals actually sense during olfactory behavior. However, reproducing the

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Treadmill Running of Mouse as a Model for Studying Influence of Maternal Exercise on Offspring

Authors: Song Ah Chae, Jun Seok Son, Mei-Jun Zhu, Jeanene M. de Avila and Min Du, date: 12/05/2020, view: 784, Q&A: 0

[Abstract]

Epidemiological studies robustly show the beneficial effects of maternal exercise in reducing maternal birth complications and improving neonatal outcomes, though underlying mechanisms remain poorly understood. To facilitate mechanistic exploration, a protocol for maternal exercise of mice is established, with the regimen following the exercise

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Visual-looming Shadow Task with in-vivo Calcium Activity Monitoring to Assess Defensive Behaviors in Mice

Authors: Nuria Daviu, Tamas Fuzesi, David G.Rosenegger, Govind Peringod, Kathryn Simone and Jaideep S. Bains, date: 11/20/2020, view: 811, Q&A: 0

[Abstract] There has been a clear movement in recent years towards the adoption of more naturalistic experimental regimes for the study of behavior and its underlying neural architecture. Here we provide a protocol that allows experimenters working with mice, to mimic a looming and advancing predatory threat from the sky. This approach is easy to implement ...

An Operant Conditioning Model Combined with a Chemogenetic Approach to Study the Neurobiology of Food Addiction in Mice

Authors: Elena Martín-García, Laura Domingo-Rodriguez and Rafael Maldonado, date: 10/05/2020, view: 772, Q&A: 0

[Abstract] The study of food addiction comprises 3 hallmarks that include the persistence to response without an outcome, the strong motivation for palatable food, and the loss of inhibitory control over food intake that leads to compulsive behavior in addicted individuals. The complex multifactorial nature of this disorder and the unknown neurobiological ...

Long-term Devocalization of Zebra Finches

Authors: Yunbok Kim, Daisuke Mizuguchi and Satoshi Kojima, date: 09/20/2020, view: 1405, Q&A: 0

[Abstract] Songbirds, such as the zebra finch, are a popular animal model for studying the neural basis of vocal and complex skill learning. Adult male zebra finches produce courtship song toward females (referred to as ‘directed song’) and recording and analyzing sounds of directed song along with underlying neural activity is important for investigating ...

The Peak Interval Procedure in Rodents: A Tool for Studying the Neurobiological Basis of Interval Timing and Its Alterations in Models of Human Disease

Authors: Fuat Balcı and David Freestone, date: 09/05/2020, view: 894, Q&A: 0

[Abstract] Animals keep track of time intervals in the seconds to minutes range with, on average, high accuracy but substantial trial-to-trial variability. The ability to detect the statistical signatures of such timing behavior is an indispensable feature of a good and theoretically-tractable testing procedure. A widely used interval timing procedure is the ...

Measuring Breathing Patterns in Mice Using Whole-body Plethysmography

Authors: Patricia Prada-Dacasa, Andrea Urpi, Laura Sánchez-Benito, Patrizia Bianchi and Albert Quintana, date: 09/05/2020, view: 1835, Q&A: 0

[Abstract] Respiratory dysfunction is among the main cause of severe and fatal pathologies worldwide. The use of effective experimental models and methodologies for the study of the pulmonary pathophysiology is necessary to prevent, control and cure these diseases. Plethysmography, a technique for the assessment of lung function, has been widely applied in ...

Sequential Reaching Task for the Study of Motor Skills in Monkeys

Authors: Machiko Ohbayashi and Nathalie Picard, date: 08/20/2020, view: 895, Q&A: 0

[Abstract] The ability to perform a sequence of movements is a key component of motor skills, such as typing or playing a musical instrument. How the brain binds elementary movements together into meaningful actions has been a topic of much interest. Here, we describe two sequential reaching tasks that we use to investigate the neural substrate of skilled ...

Multiple Simultaneous Acute Stresses in Mice: Single or Repeated Induction

Authors: Rachael E. Hokenson, Mikko Oijala, Annabel K. Short, Jessica L. Bolton, Yuncai Chen, Jenny Molet, Pamela M. Maras, Tallie Z. Baram and Gyorgy Lur, date: 08/05/2020, view: 1508, Q&A: 0

[Abstract] Stress is crucial to the survival of an organism, but excessive stress can lead to psychological disorders including depression, anxiety, substance abuse, and suicidality. The prevailing notion is that chronic stress promotes adverse outcomes on brain and body health, whereas acute stressors are generally benign. Notably, acute events such mass ...

Development of the Deoxycorticosterone Acetate (DOCA)-salt Hypertensive Rat Model

Authors: Nayara Pestana-Oliveira, David B. Nahey, Tim Johnson and John P. Collister, date: 08/05/2020, view: 1293, Q&A: 0

[Abstract] According to the World Health Organization (WHO), nearly 1.13 billion people worldwide have hypertension, a major factor responsible for premature death globally. The inherent multifactorial nature of hypertension makes its study difficult since the chronic rise in blood pressure depends on the intricate connection between dietary, genetic ...

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