Cell Biology


    Protocols in Current Issue
    Immunoprecipitation of Acetyl-lysine And Western Blotting of Long-chain acyl-CoA Dehydrogenases and Beta-hydroxyacyl-CoA Dehydrogenase in Palmitic Acid Treated Human Renal Tubular Epithelial Cells
    Authors:  Tingting Lv, Suwei Zhu, Yuan Ma, Hong Feng and Qiang Wan, date: 09/20/2020, view: 43, Q&A: 0
    [Abstract] As one of the main energy metabolism organs, kidney has been proved to have high energy requirements and are more inclined to fatty acid metabolism as the main energy source. Long-chain acyl-CoA dehydrogenases (LCAD) and beta-hydroxyacyl-CoA dehydrogenase (beta-HAD), key enzymes involved in fatty acid oxidation, has been identified as the ...
    Determination of Intracellular Ca2+ Concentration in the Human Pathogens Trypanosomatids Leishmania mexicana and Trypanosoma cruzi by the Use of the Fluorescent Ca2+ Indicator Fura-2
    Authors:  Andre Rey-Cibati, Mariana Valladares-Delgado and Gustavo Benaim, date: 09/20/2020, view: 38, Q&A: 0
    [Abstract] Ca2+ is an essential signaling messenger in all eukariotic cells, playing a pivotal role in many cellular functions as cell growth control (differentiation, fertilization and apoptosis), secretion, gene expression, enzyme regulation, among many others. This basic premise includes trypanosomatids as Trypanosoma cruzi and various ...
    An in vitro DNA Sensor-based Assay to Measure Receptor-specific Adhesion Forces of Eukaryotic Cells and Pathogens
    Authors:  Maurizio Wack, Tina Wiegand, Friedrich Frischknecht and E. Ada Cavalcanti-Adam, date: 09/05/2020, view: 720, Q&A: 0
    [Abstract] Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the molecular mechanisms driving migration. Traction force microscopy or optical tweezers are typically ...
    Quantitative Kinetic Analyses of Histone Turnover Using Imaging and Flow Cytometry
    Authors:  Hanae Sato, Robert H. Singer and John M. Greally, date: 09/05/2020, view: 612, Q&A: 0
    [Abstract] Dynamic histone changes occur as a central part of chromatin regulation. Deposition of histone variants and post-translational modifications of histones are strongly associated with properties of chromatin status. Characterizing the kinetics of histone variants allows important insights into transcription regulation, chromatin maintenance and ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    Authors:  Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon, date: 09/05/2020, view: 459, Q&A: 0
    [Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...
    Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-κB Reporter Mice
    Authors:  Artur Schmidtchen and Manoj Puthia, date: 08/20/2020, view: 809, Q&A: 0
    [Abstract] Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on ...
    In-vitro GLP-1 Release Assay Using STC-1 Cells
    Authors:  Liu Qi, Tan Shuai, Jia Da, Burstein Ezra and Sifuentes-Dominguez Luis, date: 08/20/2020, view: 590, Q&A: 0
    [Abstract] Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.
    Candida albicans Agar Invasion Assays
    Authors:  Shamoon Naseem, Lois M. Douglas and James B. Konopka, date: 08/20/2020, view: 590, Q&A: 0
    [Abstract] The ability of the human fungal pathogen Candida albicans to disseminate into tissues is promoted by a switch from budding to invasive hyphal growth. This morphological transition is stimulated by multiple environmental factors that can vary at different sites of infection. To identify genes that promote invasive growth, C. albicans ...
    Neutrophil Extracellular Trap Killing Assay of Candida albicans
    Authors:  Sheng-Yang Wu and Betty A. Wu-Hsieh, date: 08/20/2020, view: 700, Q&A: 0
    [Abstract] Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...
    Determination of the Cellular Ion Concentration in Saccharomyces cerevisiae Using ICP-AES
    Authors:  Louise Thines, Anne Iserentant and Pierre Morsomme, date: 08/20/2020, view: 593, Q&A: 0
    [Abstract] The yeast Saccharomyces cerevisiae has been perceived over decades as a highly valuable model organism for the investigation of ion homeostasis. Indeed, many of the genes and biological systems that function in yeast ion homeostasis are conserved throughout unicellular eukaryotes to humans. In this context, measurement of the yeast ...

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