Cell Biology

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    Protocols in Current Issue
    Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
    Authors:  Matthew Swire and Charles ffrench-Constant, date: 10/20/2020, view: 22, Q&A: 0
    [Abstract] Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse ...
    Double Labeling of PDGFR-β and α-SMA in Swine Models of Acute Kidney Injury to Detect Pericyte-to-Myofibroblast Transdifferentation as Early Marker of Fibrosis
    [Abstract] Growing evidences suggest that peritubular capillaries pericytes are the main source of scar-forming myofibroblasts during chronic kidney disease (CKD), as well as early phases of acute kidney injury (AKI). In a swine model of sepsis and I/R (Ischemia Reperfusion) injury-induced AKI we demonstrated that renal pericytes are able to ...
    Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples
    Authors:  Linhua Liu, Xiaojing Zhou and Jian-young Wu, date: 10/05/2020, view: 183, Q&A: 0
    [Abstract] We describe a protocol for preparing acute brain slices which can produce robust hippocampal sharp wave-ripples (SWRs) in vitro. The protocol is optimized for its simplicity and reliability for the preparation of solutions, slicing, and recovery incubation. Most slices in almost every mouse prepared though the protocol expressed vigorous ...
    Surgical Induction of Endometriosis in Female Mice
    Authors:  Alejandra Escudero-Lara, David Cabañero and Rafael Maldonado, date: 09/20/2020, view: 335, Q&A: 0
    [Abstract] Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...
    In-vitro GLP-1 Release Assay Using STC-1 Cells
    Authors:  Liu Qi, Tan Shuai, Jia Da, Burstein Ezra and Sifuentes-Dominguez Luis, date: 08/20/2020, view: 698, Q&A: 0
    [Abstract] Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.
    In vivo Blood-brain Barrier Permeability Assays Using Clostridium perfringens Epsilon Toxin
    Authors:  Michael R. Mazzucco, Timothy Vartanian and Jennifer R. Linden, date: 08/05/2020, view: 687, Q&A: 0
    [Abstract] In order for the brain to function properly, a carefully orchestrated homeostasis must be maintained. To help regulate this delicate balance, the brain has developed a highly selective blood-brain barrier (BBB). Under normal conditions, the BBB excludes harmful blood-borne material from the brain parenchyma. However, numerous neuropathological ...
    Mechanical Tissue Compression and Whole-mount Imaging at Single Cell Resolution for Developing Murine Epididymal Tubules
    Author:  Tsuyoshi Hirashima, date: 08/05/2020, view: 876, Q&A: 0
    [Abstract] Cells inside the body are subjected to various mechanical stress, such as stretch or compression provided by surrounding cells, shear stresses by blood or lymph flows, and normal stresses by luminal liquids. Force loading to the biological tissues is a fundamental method to better understand cellular responses to such mechanical stimuli. There ...
    Development of the Deoxycorticosterone Acetate (DOCA)-salt Hypertensive Rat Model
    Authors:  Nayara Pestana-Oliveira, David B. Nahey, Tim Johnson and John P. Collister, date: 08/05/2020, view: 509, Q&A: 0
    [Abstract] According to the World Health Organization (WHO), nearly 1.13 billion people worldwide have hypertension, a major factor responsible for premature death globally. The inherent multifactorial nature of hypertension makes its study difficult since the chronic rise in blood pressure depends on the intricate connection between dietary, genetic ...
    Method for Prolonged Incubation of Brain Slices
    Authors:  Orsolya Kekesi and Yossi Buskila, date: 07/20/2020, view: 539, Q&A: 0
    [Abstract] Slices of neuronal tissue maintain a high degree of topographical and functional properties of neurons and glia and therefore are extensively used for measurements of neuronal activity at the molecular, cellular and network levels. However, the lifespan of slice preparations is narrow, averaging of 6-8 hours. Moreover, the average viability of ...
    Analysis of Generalized Fibrosis in Mouse Tissue Sections with Masson’s Trichrome Staining
    Authors:  Diantha van de Vlekkert, Eda Machado and Alessandra d’Azzo, date: 05/20/2020, view: 1140, Q&A: 0
    [Abstract] Expansion of fibrous connective tissue and abnormal deposition of extracellular matrix (ECM) are at the basis of many fibrotic diseases. Fibrosis can occur in response to both physiological and pathological cues, including wound healing, tissue remodeling/repair and inflammation. Chronic fibrosis can lead to severe tissue damage, organ failure and ...



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