Cell Biology

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    Protocols in Current Issue
    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    Authors:  Isha Rana, Krithika Badarinath, Ravindra K. Zirmire and Colin Jamora, date: 09/05/2021, view: 709, Q&A: 0
    [Abstract]

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis. The deregulation of this crosstalk can lead to abnormal healing processes and is the foundation of

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    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    Authors:  Longjun Li, Rudi Mao and Xiaopeng Qi, date: 09/05/2021, view: 605, Q&A: 0
    [Abstract]

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), cytoplasmic sensor protein, and the effector protein pro-caspase-1. ASC assembly into a protein

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    A Novel Method to Map Small RNAs with High Resolution
    Authors:  Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan, date: 08/20/2021, view: 535, Q&A: 0
    [Abstract]

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics in situ. However, the application of super-resolution imaging techniques to detect

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    CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes
    Authors:  Masahito Yamagata and Joshua R. Sanes, date: 08/05/2021, view: 1190, Q&A: 0
    [Abstract]

    The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis; in other species, other methods are needed to selectively label somatic cells based on the genes

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    Spatiotemporal Quantification of Cytosolic pH in Arabidopsis Pollen Tubes
    Authors:  Maria Teresa Portes, Daniel S.C. Damineli and José A. Feijó, date: 07/20/2021, view: 796, Q&A: 0
    [Abstract]

    Ion-specific probes and fluorescent indicators have been key in establishing the role of ion signaling, namely calcium, protons, and anions, in plant development, providing a robust approach for monitoring spatiotemporal changes in intracellular ion dynamics. The integration of protons/pH in signaling mechanisms is especially important as reports

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    A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
    Authors:  Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich, date: 07/20/2021, view: 1118, Q&A: 0
    [Abstract]

    Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This technique employs a pair of complementary intrabodies loaded into living cells that co-translationally bind

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    A Method for Estimating the Potential Synaptic Connections Between Axons and Dendrites From 2D Neuronal Images
    Authors:  Carolina Tecuatl, Diek W. Wheeler and Giorgio A. Ascoli, date: 07/05/2021, view: 1560, Q&A: 0
    [Abstract]

    Computational neuroscience aims to model, reproduce, and predict network dynamics for different neuronal ensembles by distilling knowledge derived from electrophysiological and morphological evidence. However, analyses and simulations often remain critically limited by the sparsity of direct experimental constraints on essential parameters, such

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    Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
    Authors:  Henrik Renner, Mandy Otto, Martha Grabos, Hans R. Schöler and Jan M. Bruder, date: 06/20/2021, view: 1466, Q&A: 1
    [Abstract]

    Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning is the standard method for immunohistochemical analysis. However, it removes cells

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    In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium
    Authors:  Shigenori Inagaki, Ryo Iwata and Takeshi Imai, date: 06/20/2021, view: 1192, Q&A: 0
    [Abstract]

    In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant receptors (ORs) that are expressed by olfactory sensory neurons (OSNs). Since each OSN expresses only

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    Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
    Authors:  Yoko Bekku and James L. Salzer, date: 06/20/2021, view: 1067, Q&A: 0
    [Abstract]

    The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from a combination of selective targeting and retention of membrane proteins. In neurons, most proteins

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